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Functional expression of a MAP kinase kinase in COS cells and recognition by an anti-STE7/byr1 antibody.

Publication ,  Journal Article
Haystead, CM; Wu, J; Gregory, P; Sturgill, TW; Haystead, TA
Published in: FEBS Lett
February 8, 1993

Mitogen-activated protein (MAP) kinases p42mapk and p44mapk are activated by dual tyrosine and threonine phosphorylation in vivo. Both MAPKs are phosphorylated and activated in vitro by an activator recently identified as a protein-tyrosine/threonine kinase. We have isolated a putative cDNA for a MAP kinase kinase (MAPKK) and determined its structure [Proc. Natl. Acad. Sci. USA, in press]. The protein encoded by this cDNA shares sequence homology with two yeast protein kinases byr1 and STE7. We now report that stimulation with serum of COS cells expressing this shares sequence homology with two yeast protein kinases byr1 and STE7. We now report that stimulation with serum of COS cells expressing this protein amplifies MAPK activator activity markedly. The increased activity co-migrates during chromatography with the expressed 45 kDa protein, recognized by an anti-STE7/byr1 antibody, and is abrogated by treatment with phosphatase 2A. Thus, this cDNA encodes a functional MAPKK. The anti-STE7/byr1 antibody also recognized a 46 kDa COS cell protein that was resolved from the expressed MAPKK by anion-exchange chromatography. This immunoreactive protein co-eluted with endogenous MAPKK activity, suggesting identification of the immunoreactive band as monkey MAPKK.

Duke Scholars

Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

February 8, 1993

Volume

317

Issue

1-2

Start / End Page

12 / 16

Location

England

Related Subject Headings

  • Rats
  • Protein Kinases
  • Phosphorylation
  • Molecular Sequence Data
  • Mitogen-Activated Protein Kinase Kinases
  • Kidney
  • DNA
  • Cloning, Molecular
  • Cells, Cultured
  • Biochemistry & Molecular Biology
 

Citation

APA
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ICMJE
MLA
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Haystead, C. M., Wu, J., Gregory, P., Sturgill, T. W., & Haystead, T. A. (1993). Functional expression of a MAP kinase kinase in COS cells and recognition by an anti-STE7/byr1 antibody. FEBS Lett, 317(1–2), 12–16. https://doi.org/10.1016/0014-5793(93)81481-e
Haystead, C. M., J. Wu, P. Gregory, T. W. Sturgill, and T. A. Haystead. “Functional expression of a MAP kinase kinase in COS cells and recognition by an anti-STE7/byr1 antibody.FEBS Lett 317, no. 1–2 (February 8, 1993): 12–16. https://doi.org/10.1016/0014-5793(93)81481-e.
Haystead CM, Wu J, Gregory P, Sturgill TW, Haystead TA. Functional expression of a MAP kinase kinase in COS cells and recognition by an anti-STE7/byr1 antibody. FEBS Lett. 1993 Feb 8;317(1–2):12–6.
Haystead, C. M., et al. “Functional expression of a MAP kinase kinase in COS cells and recognition by an anti-STE7/byr1 antibody.FEBS Lett, vol. 317, no. 1–2, Feb. 1993, pp. 12–16. Pubmed, doi:10.1016/0014-5793(93)81481-e.
Haystead CM, Wu J, Gregory P, Sturgill TW, Haystead TA. Functional expression of a MAP kinase kinase in COS cells and recognition by an anti-STE7/byr1 antibody. FEBS Lett. 1993 Feb 8;317(1–2):12–16.
Journal cover image

Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

February 8, 1993

Volume

317

Issue

1-2

Start / End Page

12 / 16

Location

England

Related Subject Headings

  • Rats
  • Protein Kinases
  • Phosphorylation
  • Molecular Sequence Data
  • Mitogen-Activated Protein Kinase Kinases
  • Kidney
  • DNA
  • Cloning, Molecular
  • Cells, Cultured
  • Biochemistry & Molecular Biology