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Insulin and diabetes cause reciprocal changes in the association of eIF-4E and PHAS-I in rat skeletal muscle.

Publication ,  Journal Article
Kimball, SR; Jefferson, LS; Fadden, P; Haystead, TA; Lawrence, JC
Published in: Am J Physiol
February 1996

We have investigated the roles of eukaryotic initiation factor 4E (eIF-4E), the cap-binding protein, and the translational regulator, PHAS-I, in the effects of insulin and alloxan-induced diabetes on protein synthesis in rat skeletal muscle. Diabetes increased the amount of eIF-4E found in the inactive PHAS-I.eIF-4E complex by threefold, explaining in part the inhibitory effect of insulin deficiency on translation initiation. Insulin treatment of diabetic rats caused dissociation of the complex, consistent with the action of the hormone on reversing the inhibitory effect of diabetes on translation initiation. The effects of both insulin and diabetes on PHAS-I binding to eIF-4E appeared to be due to changes in PHAS-I phosphorylation. Neither insulin nor diabetes changed the phosphorylation state of eIF-4E. The results indicate that the effects of both insulin and diabetes on protein synthesis in skeletal muscle involve modulation of the interaction of PHAS-I and eIF-4E.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

February 1996

Volume

270

Issue

2 Pt 1

Start / End Page

C705 / C709

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Phosphorylation
  • Phosphoproteins
  • Peptide Initiation Factors
  • Muscle, Skeletal
  • Male
  • Intracellular Signaling Peptides and Proteins
  • Insulin
  • Eukaryotic Initiation Factor-4E
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kimball, S. R., Jefferson, L. S., Fadden, P., Haystead, T. A., & Lawrence, J. C. (1996). Insulin and diabetes cause reciprocal changes in the association of eIF-4E and PHAS-I in rat skeletal muscle. Am J Physiol, 270(2 Pt 1), C705–C709. https://doi.org/10.1152/ajpcell.1996.270.2.C705
Kimball, S. R., L. S. Jefferson, P. Fadden, T. A. Haystead, and J. C. Lawrence. “Insulin and diabetes cause reciprocal changes in the association of eIF-4E and PHAS-I in rat skeletal muscle.Am J Physiol 270, no. 2 Pt 1 (February 1996): C705–9. https://doi.org/10.1152/ajpcell.1996.270.2.C705.
Kimball SR, Jefferson LS, Fadden P, Haystead TA, Lawrence JC. Insulin and diabetes cause reciprocal changes in the association of eIF-4E and PHAS-I in rat skeletal muscle. Am J Physiol. 1996 Feb;270(2 Pt 1):C705–9.
Kimball, S. R., et al. “Insulin and diabetes cause reciprocal changes in the association of eIF-4E and PHAS-I in rat skeletal muscle.Am J Physiol, vol. 270, no. 2 Pt 1, Feb. 1996, pp. C705–09. Pubmed, doi:10.1152/ajpcell.1996.270.2.C705.
Kimball SR, Jefferson LS, Fadden P, Haystead TA, Lawrence JC. Insulin and diabetes cause reciprocal changes in the association of eIF-4E and PHAS-I in rat skeletal muscle. Am J Physiol. 1996 Feb;270(2 Pt 1):C705–C709.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

February 1996

Volume

270

Issue

2 Pt 1

Start / End Page

C705 / C709

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Phosphorylation
  • Phosphoproteins
  • Peptide Initiation Factors
  • Muscle, Skeletal
  • Male
  • Intracellular Signaling Peptides and Proteins
  • Insulin
  • Eukaryotic Initiation Factor-4E