Dual Ser and Thr phosphorylation of CPI-17, an inhibitor of myosin phosphatase, by MYPT-associated kinase.
Journal Article (Journal Article)
Phosphorylation of CPI-17 and PHI-1 by the MYPT1-associated kinase (M110 kinase) was investigated. M110 kinase is a recently identified serine/threonine kinase with a catalytic domain that is homologous to that of ZIP kinase (ZIPK. GST-rN-ZIPK, a constitutively active GST fusion fragment, phosphorylates CPI-17 (but not PHI-1) to a stoichiometry of 1.7 mol/mol. Phosphoamino acid analysis revealed phosphorylation of both Ser and Thr residues. Phosphorylation sites in CPI-17 were identified as Thr 38 and Ser 12 using Edman sequencing with (32)P release and a point mutant of Thr 38.
Full Text
Duke Authors
Cited Authors
- MacDonald, JA; Eto, M; Borman, MA; Brautigan, DL; Haystead, TA
Published Date
- March 30, 2001
Published In
Volume / Issue
- 493 / 2-3
Start / End Page
- 91 - 94
PubMed ID
- 11287002
International Standard Serial Number (ISSN)
- 0014-5793
Digital Object Identifier (DOI)
- 10.1016/s0014-5793(01)02277-3
Language
- eng
Conference Location
- England