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Dual Ser and Thr phosphorylation of CPI-17, an inhibitor of myosin phosphatase, by MYPT-associated kinase.

Publication ,  Journal Article
MacDonald, JA; Eto, M; Borman, MA; Brautigan, DL; Haystead, TA
Published in: FEBS Lett
March 30, 2001

Phosphorylation of CPI-17 and PHI-1 by the MYPT1-associated kinase (M110 kinase) was investigated. M110 kinase is a recently identified serine/threonine kinase with a catalytic domain that is homologous to that of ZIP kinase (ZIPK. GST-rN-ZIPK, a constitutively active GST fusion fragment, phosphorylates CPI-17 (but not PHI-1) to a stoichiometry of 1.7 mol/mol. Phosphoamino acid analysis revealed phosphorylation of both Ser and Thr residues. Phosphorylation sites in CPI-17 were identified as Thr 38 and Ser 12 using Edman sequencing with (32)P release and a point mutant of Thr 38.

Duke Scholars

Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

March 30, 2001

Volume

493

Issue

2-3

Start / End Page

91 / 94

Location

England

Related Subject Headings

  • Threonine
  • Serine
  • Recombinant Fusion Proteins
  • Protein Serine-Threonine Kinases
  • Point Mutation
  • Phosphorylation
  • Phosphoproteins
  • Phosphoprotein Phosphatases
  • Myosin-Light-Chain Phosphatase
  • Muscle Proteins
 

Citation

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MacDonald, J. A., Eto, M., Borman, M. A., Brautigan, D. L., & Haystead, T. A. (2001). Dual Ser and Thr phosphorylation of CPI-17, an inhibitor of myosin phosphatase, by MYPT-associated kinase. FEBS Lett, 493(2–3), 91–94. https://doi.org/10.1016/s0014-5793(01)02277-3
MacDonald, J. A., M. Eto, M. A. Borman, D. L. Brautigan, and T. A. Haystead. “Dual Ser and Thr phosphorylation of CPI-17, an inhibitor of myosin phosphatase, by MYPT-associated kinase.FEBS Lett 493, no. 2–3 (March 30, 2001): 91–94. https://doi.org/10.1016/s0014-5793(01)02277-3.
MacDonald JA, Eto M, Borman MA, Brautigan DL, Haystead TA. Dual Ser and Thr phosphorylation of CPI-17, an inhibitor of myosin phosphatase, by MYPT-associated kinase. FEBS Lett. 2001 Mar 30;493(2–3):91–4.
MacDonald, J. A., et al. “Dual Ser and Thr phosphorylation of CPI-17, an inhibitor of myosin phosphatase, by MYPT-associated kinase.FEBS Lett, vol. 493, no. 2–3, Mar. 2001, pp. 91–94. Pubmed, doi:10.1016/s0014-5793(01)02277-3.
MacDonald JA, Eto M, Borman MA, Brautigan DL, Haystead TA. Dual Ser and Thr phosphorylation of CPI-17, an inhibitor of myosin phosphatase, by MYPT-associated kinase. FEBS Lett. 2001 Mar 30;493(2–3):91–94.
Journal cover image

Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

March 30, 2001

Volume

493

Issue

2-3

Start / End Page

91 / 94

Location

England

Related Subject Headings

  • Threonine
  • Serine
  • Recombinant Fusion Proteins
  • Protein Serine-Threonine Kinases
  • Point Mutation
  • Phosphorylation
  • Phosphoproteins
  • Phosphoprotein Phosphatases
  • Myosin-Light-Chain Phosphatase
  • Muscle Proteins