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CRP: Cleavage of Radiolabeled Phosphoproteins.

Publication ,  Journal Article
Mackey, AJ; Haystead, TAJ; Pearson, WR
Published in: Nucleic Acids Res
July 1, 2003

The CRP (Cleavage of Radiolabeled Phosphoproteins) program guides the design and interpretation of experiments to identify protein phosphorylation sites by Edman sequencing of unseparated peptides. Traditionally, phosphorylation sites are determined by cleaving the phosphoprotein and separating the peptides for Edman 32P-phosphate release sequencing. CRP analysis of a phosphoprotein's sequence accelerates this process by omitting the separation step: given a protein sequence of interest, the CRP program performs an in silico proteolytic cleavage of the sequence and reports the predicted Edman cycles in which radioactivity would be observed if a given serine, threonine or tyrosine were phosphorylated. Experimentally observed cycles containing 32P can be compared with CRP predictions to confirm candidate sites and/or explore the ability of additional cleavage experiments to resolve remaining ambiguities. To reduce ambiguity, the phosphorylated residue (P-Tyr, P-Ser or P-Thr) can be determined experimentally, and CRP will ignore sites with alternative residues. CRP also provides simple predictions of likely phosphorylation sites using known kinase recognition motifs. The CRP interface is available at http://fasta.bioch.virginia.edu/crp.

Duke Scholars

Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

July 1, 2003

Volume

31

Issue

13

Start / End Page

3859 / 3861

Location

England

Related Subject Headings

  • Software
  • Sequence Analysis, Protein
  • Radioactive Tracers
  • Phosphotyrosine
  • Phosphothreonine
  • Phosphoserine
  • Phosphorylation
  • Phosphoproteins
  • Internet
  • Humans
 

Citation

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Mackey, A. J., Haystead, T. A. J., & Pearson, W. R. (2003). CRP: Cleavage of Radiolabeled Phosphoproteins. Nucleic Acids Res, 31(13), 3859–3861. https://doi.org/10.1093/nar/gkg513
Mackey, Aaron J., Timothy A. J. Haystead, and William R. Pearson. “CRP: Cleavage of Radiolabeled Phosphoproteins.Nucleic Acids Res 31, no. 13 (July 1, 2003): 3859–61. https://doi.org/10.1093/nar/gkg513.
Mackey AJ, Haystead TAJ, Pearson WR. CRP: Cleavage of Radiolabeled Phosphoproteins. Nucleic Acids Res. 2003 Jul 1;31(13):3859–61.
Mackey, Aaron J., et al. “CRP: Cleavage of Radiolabeled Phosphoproteins.Nucleic Acids Res, vol. 31, no. 13, July 2003, pp. 3859–61. Pubmed, doi:10.1093/nar/gkg513.
Mackey AJ, Haystead TAJ, Pearson WR. CRP: Cleavage of Radiolabeled Phosphoproteins. Nucleic Acids Res. 2003 Jul 1;31(13):3859–3861.
Journal cover image

Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

July 1, 2003

Volume

31

Issue

13

Start / End Page

3859 / 3861

Location

England

Related Subject Headings

  • Software
  • Sequence Analysis, Protein
  • Radioactive Tracers
  • Phosphotyrosine
  • Phosphothreonine
  • Phosphoserine
  • Phosphorylation
  • Phosphoproteins
  • Internet
  • Humans