Delineating signal transduction pathways in smooth muscle through focused proteomics.


Journal Article (Review)

This review will outline examples of the authors' focused proteomics approaches to studying signal transduction pathways in smooth muscle. By focusing the use of traditional proteomics techniques with hypothesis-driven selection methods, this approach efficiently addresses the identification of novel elements in a signal transduction pathway of interest. However, focused proteomics serves only as a starting point in the investigation of novel signaling proteins. While focused proteomics studies can suggest the involvement and general biochemical function of a protein in a signaling pathway, these findings must be further investigated and validated. Through the integrated use of focused proteomics with complementary approaches such as genetics, biochemistry and cell physiology, a complete and detailed mechanism of signal transduction can be determined.

Full Text

Duke Authors

Cited Authors

  • Hagerty, L; Haystead, TAJ

Published Date

  • February 2006

Published In

Volume / Issue

  • 3 / 1

Start / End Page

  • 75 - 85

PubMed ID

  • 16445352

Pubmed Central ID

  • 16445352

Electronic International Standard Serial Number (EISSN)

  • 1744-8387

Digital Object Identifier (DOI)

  • 10.1586/14789450.3.1.75


  • eng

Conference Location

  • England