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Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose.

Publication ,  Journal Article
Moorhead, GBG; Haystead, TAJ; MacKintosh, C
Published in: Methods Mol Biol
2007

Microcystin-based affinity matrices have been utilized to demonstrate the association of signaling proteins with protein phosphatases and for the purification of low-abundance microcystin-sensitive protein phosphatases. Here, we describe the procedure for the synthesis and use of microcystin-Sepharose and microcystin-biotin-Sepharose.

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Published In

Methods Mol Biol

DOI

ISSN

1064-3745

Publication Date

2007

Volume

365

Start / End Page

39 / 45

Location

United States

Related Subject Headings

  • Sepharose
  • Reproducibility of Results
  • Phosphoprotein Phosphatases
  • Peptides, Cyclic
  • Microcystins
  • Developmental Biology
  • Chromatography, Affinity
  • Biotin
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences
 

Citation

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Moorhead, G. B. G., Haystead, T. A. J., & MacKintosh, C. (2007). Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose. Methods Mol Biol, 365, 39–45. https://doi.org/10.1385/1-59745-267-X:39
Moorhead, Greg B. G., Timothy A. J. Haystead, and Carol MacKintosh. “Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose.Methods Mol Biol 365 (2007): 39–45. https://doi.org/10.1385/1-59745-267-X:39.
Moorhead, Greg B. G., et al. “Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose.Methods Mol Biol, vol. 365, 2007, pp. 39–45. Pubmed, doi:10.1385/1-59745-267-X:39.

Published In

Methods Mol Biol

DOI

ISSN

1064-3745

Publication Date

2007

Volume

365

Start / End Page

39 / 45

Location

United States

Related Subject Headings

  • Sepharose
  • Reproducibility of Results
  • Phosphoprotein Phosphatases
  • Peptides, Cyclic
  • Microcystins
  • Developmental Biology
  • Chromatography, Affinity
  • Biotin
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences