Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose.
Publication
, Journal Article
Moorhead, GBG; Haystead, TAJ; MacKintosh, C
Published in: Methods Mol Biol
2007
Microcystin-based affinity matrices have been utilized to demonstrate the association of signaling proteins with protein phosphatases and for the purification of low-abundance microcystin-sensitive protein phosphatases. Here, we describe the procedure for the synthesis and use of microcystin-Sepharose and microcystin-biotin-Sepharose.
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Published In
Methods Mol Biol
DOI
ISSN
1064-3745
Publication Date
2007
Volume
365
Start / End Page
39 / 45
Location
United States
Related Subject Headings
- Sepharose
- Reproducibility of Results
- Phosphoprotein Phosphatases
- Peptides, Cyclic
- Microcystins
- Developmental Biology
- Chromatography, Affinity
- Biotin
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Moorhead, G. B. G., Haystead, T. A. J., & MacKintosh, C. (2007). Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose. Methods Mol Biol, 365, 39–45. https://doi.org/10.1385/1-59745-267-X:39
Moorhead, Greg B. G., Timothy A. J. Haystead, and Carol MacKintosh. “Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose.” Methods Mol Biol 365 (2007): 39–45. https://doi.org/10.1385/1-59745-267-X:39.
Moorhead GBG, Haystead TAJ, MacKintosh C. Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose. Methods Mol Biol. 2007;365:39–45.
Moorhead, Greg B. G., et al. “Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose.” Methods Mol Biol, vol. 365, 2007, pp. 39–45. Pubmed, doi:10.1385/1-59745-267-X:39.
Moorhead GBG, Haystead TAJ, MacKintosh C. Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose. Methods Mol Biol. 2007;365:39–45.
Published In
Methods Mol Biol
DOI
ISSN
1064-3745
Publication Date
2007
Volume
365
Start / End Page
39 / 45
Location
United States
Related Subject Headings
- Sepharose
- Reproducibility of Results
- Phosphoprotein Phosphatases
- Peptides, Cyclic
- Microcystins
- Developmental Biology
- Chromatography, Affinity
- Biotin
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences