Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose.

Journal Article

Microcystin-based affinity matrices have been utilized to demonstrate the association of signaling proteins with protein phosphatases and for the purification of low-abundance microcystin-sensitive protein phosphatases. Here, we describe the procedure for the synthesis and use of microcystin-Sepharose and microcystin-biotin-Sepharose.

Full Text

Duke Authors

Cited Authors

  • Moorhead, GBG; Haystead, TAJ; MacKintosh, C

Published Date

  • 2007

Published In

Volume / Issue

  • 365 /

Start / End Page

  • 39 - 45

PubMed ID

  • 17200552

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1385/1-59745-267-X:39

Language

  • eng

Conference Location

  • United States