Metabolic regulation of oocyte cell death through the CaMKII-mediated phosphorylation of caspase-2.

Journal Article (Journal Article)

Vertebrate female reproduction is limited by the oocyte stockpiles acquired during embryonic development. These are gradually depleted over the organism's lifetime through the process of apoptosis. The timer that triggers this cell death is yet to be identified. We used the Xenopus egg/oocyte system to examine the hypothesis that nutrient stores can regulate oocyte viability. We show that pentose-phosphate-pathway generation of NADPH is critical for oocyte survival and that the target of this regulation is caspase-2, previously shown to be required for oocyte death in mice. Pentose-phosphate-pathway-mediated inhibition of cell death was due to the inhibitory phosphorylation of caspase-2 by calcium/calmodulin-dependent protein kinase II (CaMKII). These data suggest that exhaustion of oocyte nutrients, resulting in an inability to generate NADPH, may contribute to ooctye apoptosis. These data also provide unexpected links between oocyte metabolism, CaMKII, and caspase-2.

Full Text

Duke Authors

Cited Authors

  • Nutt, LK; Margolis, SS; Jensen, M; Herman, CE; Dunphy, WG; Rathmell, JC; Kornbluth, S

Published Date

  • October 7, 2005

Published In

Volume / Issue

  • 123 / 1

Start / End Page

  • 89 - 103

PubMed ID

  • 16213215

Pubmed Central ID

  • PMC2788768

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/j.cell.2005.07.032


  • eng

Conference Location

  • United States