Cytokine stimulation of aerobic glycolysis in hematopoietic cells exceeds proliferative demand.


Journal Article

The relationship between growth factor-dependent cell growth and proliferation and the up-regulation of cellular metabolism required to support these processes remains poorly defined. Here, we demonstrate that cell growth, proliferation, and glucose metabolism are coordinately regulated by interleukin-3 (IL-3) in cytokine-dependent cells. Surprisingly, glycolytic activity is stimulated to a greater extent than would be expected based on the rate of cell growth or proliferation. IL-3 signaling exerts a direct effect on glycolytic commitment independent of cell growth control. These results are not restricted to IL-3 as the cytokines IL-7 and IL-2 have similar effects on glucose metabolism when assayed in factor-dependent cell lines or primary lymphocytes, respectively. Growth factor stimulation leads cells to consume less oxygen and produce more lactate per glucose, indicative of conversion from oxidative to glycolytic metabolism. The enforced rate of glucose metabolism is in excess of that required to support cell growth; accordingly, if extracellular glucose is reduced, cells retain the ability to grow and proliferate by derepressing oxidative metabolism. These data suggest that the high rate of glycolysis observed in response to growth factor stimulation is a primary effect rather than a homeostatic response to increased cell growth.

Full Text

Duke Authors

Cited Authors

  • Bauer, DE; Harris, MH; Plas, DR; Lum, JJ; Hammerman, PS; Rathmell, JC; Riley, JL; Thompson, CB

Published Date

  • August 2004

Published In

Volume / Issue

  • 18 / 11

Start / End Page

  • 1303 - 1305

PubMed ID

  • 15180958

Pubmed Central ID

  • 15180958

Electronic International Standard Serial Number (EISSN)

  • 1530-6860

Digital Object Identifier (DOI)

  • 10.1096/fj.03-1001fje


  • eng

Conference Location

  • United States