Skip to main content

Specific role for protein kinase C beta in cell differentiation.

Publication ,  Journal Article
Gamard, CJ; Blobe, GC; Hannun, YA; Obeid, LM
Published in: Cell Growth Differ
April 1994

A critical role for protein kinase C (PKC) in signal transduction events has been well established. On the other hand, emerging evidence also suggests a role for regulation of PKC levels in mediating long term cellular functions. In human leukemia cell line HL-60, the action of 1,25-dihydroxyvitamin D3 results in transcriptional up-regulation of PKC beta (within 8-12 h) (L. M. Obeid et al., J. Biol. Chem., 265: 2370-2374, 1990). In this study, the role of PKC beta in the regulation of proliferation and differentiation was studied. 1,25-Dihydroxyvitamin D3 caused an increase in PKC beta I, beta II, and, to a lesser extent, PKC alpha, as determined by Western blot analysis. This increase was accompanied by inhibition of proliferation and induction of differentiation. The addition of a 25-base pair antisense oligonucleotide directed against the 5' coding sequence of PKC beta attenuated up-regulation of PKC beta I and beta II levels in response to 1,25-dihydroxyvitamin D3. This antisense oligonucleotide, but not sense oligonucleotide or antisense oligonucleotide to PKC alpha, caused inhibition of 1,25-dihydroxyvitamin D3-induced differentiation by 25-45%. On the other hand, inhibition of cell proliferation by 1,25-dihydroxyvitamin D3 was minimally affected by the addition of antisense oligonucleotides. These results identify a role for PKC beta in cell differentiation and underscore the significance of transcriptional activation of PKC as a mechanism for long-term regulation of PKC. The results also distinguish signaling pathways involved in cell differentiation from those involved in antiproliferation.

Duke Scholars

Published In

Cell Growth Differ

ISSN

1044-9523

Publication Date

April 1994

Volume

5

Issue

4

Start / End Page

405 / 409

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Protein Kinase C beta
  • Protein Kinase C
  • Oncology & Carcinogenesis
  • Oligonucleotides, Antisense
  • Molecular Sequence Data
  • Leukemia, Promyelocytic, Acute
  • Humans
  • Developmental Biology
  • Cell Differentiation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Gamard, C. J., Blobe, G. C., Hannun, Y. A., & Obeid, L. M. (1994). Specific role for protein kinase C beta in cell differentiation. Cell Growth Differ, 5(4), 405–409.
Gamard, C. J., G. C. Blobe, Y. A. Hannun, and L. M. Obeid. “Specific role for protein kinase C beta in cell differentiation.Cell Growth Differ 5, no. 4 (April 1994): 405–9.
Gamard CJ, Blobe GC, Hannun YA, Obeid LM. Specific role for protein kinase C beta in cell differentiation. Cell Growth Differ. 1994 Apr;5(4):405–9.
Gamard, C. J., et al. “Specific role for protein kinase C beta in cell differentiation.Cell Growth Differ, vol. 5, no. 4, Apr. 1994, pp. 405–09.
Gamard CJ, Blobe GC, Hannun YA, Obeid LM. Specific role for protein kinase C beta in cell differentiation. Cell Growth Differ. 1994 Apr;5(4):405–409.

Published In

Cell Growth Differ

ISSN

1044-9523

Publication Date

April 1994

Volume

5

Issue

4

Start / End Page

405 / 409

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Protein Kinase C beta
  • Protein Kinase C
  • Oncology & Carcinogenesis
  • Oligonucleotides, Antisense
  • Molecular Sequence Data
  • Leukemia, Promyelocytic, Acute
  • Humans
  • Developmental Biology
  • Cell Differentiation