CD22 regulates B lymphocyte function in vivo through both ligand-dependent and ligand-independent mechanisms.

Published

Journal Article

The interaction of CD22 with alpha2,6-linked sialic acid ligands has been widely proposed to regulate B lymphocyte function and migration. Here, we generated gene-targeted mice that express mutant CD22 molecules that do not interact with these ligands. CD22 ligand binding regulated the expression of cell surface CD22, immunoglobulin M and major histocompatibility complex class II on mature B cells, maintenance of the marginal zone B cell population, optimal B cell antigen receptor-induced proliferation, and B cell turnover rates. However, CD22 negative regulation of calcium mobilization after B cell antigen receptor ligation, CD22 phosphorylation, recruitment of SHP-1 to CD22 and B cell migration did not require CD22 ligand engagement. These observations resolve longstanding questions regarding the physiological importance of CD22 ligand binding in the regulation of B cell function in vivo.

Full Text

Duke Authors

Cited Authors

  • Poe, JC; Fujimoto, Y; Hasegawa, M; Haas, KM; Miller, AS; Sanford, IG; Bock, CB; Fujimoto, M; Tedder, TF

Published Date

  • October 2004

Published In

Volume / Issue

  • 5 / 10

Start / End Page

  • 1078 - 1087

PubMed ID

  • 15378059

Pubmed Central ID

  • 15378059

International Standard Serial Number (ISSN)

  • 1529-2908

Digital Object Identifier (DOI)

  • 10.1038/ni1121

Language

  • eng

Conference Location

  • United States