Cutting edge: C3d functions as a molecular adjuvant in the absence of CD21/35 expression.

Published

Journal Article

Complement component C3 covalently attaches to Ags following activation, where the C3d cleavage fragment can function as a molecular adjuvant to augment humoral immune responses. C3d is proposed to exert its adjuvant-like activities by targeting Ags to the C3d receptor (CD21/35) expressed by B cells and follicular dendritic cells. To directly assess the importance of CD21/35 in mediating the immunostimulatory effects of C3d, CD21/35-deficient (CD21/35(-/-)) mice were immunized with streptavidin (SA), SA-C3dg tetramers, recombinant HIV gp120 (gp120), or gp120 fused with linear multimers of C3d. Remarkably, SA- and gp120-specific Ab responses were significantly augmented in CD21/35(-/-) mice when these Ags were complexed with C3d in comparison to Ag alone. In fact, primary and secondary Ab responses and Ab-forming cell responses of CD21/35(-/-) mice approached those of wild-type mice immunized with SA-C3dg and gp120-C3d. Thus, C3d can function as a molecular adjuvant in the absence of CD21/35 expression.

Full Text

Duke Authors

Cited Authors

  • Haas, KM; Toapanta, FR; Oliver, JA; Poe, JC; Weis, JH; Karp, DR; Bower, JF; Ross, TM; Tedder, TF

Published Date

  • May 15, 2004

Published In

Volume / Issue

  • 172 / 10

Start / End Page

  • 5833 - 5837

PubMed ID

  • 15128761

Pubmed Central ID

  • 15128761

International Standard Serial Number (ISSN)

  • 0022-1767

Digital Object Identifier (DOI)

  • 10.4049/jimmunol.172.10.5833

Language

  • eng

Conference Location

  • United States