Barbiturate and picrotoxin-sensitive chloride efflux in rat cerebral cortical synaptoneurosomes.

Published

Journal Article

The effects of various barbiturates and picrotoxin in modifying the efflux of chloride (36Cl-) was studied in a novel subcellular preparation from rat cerebral cortex, the 'synaptoneurosome'. Dilution of synaptoneurosomes pre-loaded with 36Cl- resulted in rapid efflux of 36Cl- that could be measured as early as 10 s following dilution. In the presence of barbiturates such as pentobarbital and hexobarbital there was a significant increase in 36Cl- efflux which was not observed with the pharmacologically-inactive barbiturate, barbital. The effect of barbiturates in enhancing 36Cl- efflux was also stereospecific [(-)-DMBB greater than (+)-DMBB] and reversed by picrotoxin. By contrast, picrotoxin alone significantly inhibited 36Cl- efflux. These data demonstrate pharmacologically relevant Cl- transport for the first time in a subcellular brain preparation.

Full Text

Duke Authors

Cited Authors

  • Schwartz, RD; Skolnick, P; Hollingsworth, EB; Paul, SM

Published Date

  • September 17, 1984

Published In

Volume / Issue

  • 175 / 1

Start / End Page

  • 193 - 196

PubMed ID

  • 6479335

Pubmed Central ID

  • 6479335

International Standard Serial Number (ISSN)

  • 0014-5793

Digital Object Identifier (DOI)

  • 10.1016/0014-5793(84)80597-9

Language

  • eng

Conference Location

  • England