Control of simian virus 40 DNA replication by the HeLa cell nuclear kinase casein kinase I.

Journal Article (Journal Article)

The initiation of simian virus 40 (SV40) DNA replication is regulated by the phosphorylation state of the viral initiator protein, large T antigen. We describe the purification from HeLa cell nuclei of a 35-kDa serine/threonine protein kinase that phosphorylates T antigen at sites that are phosphorylated in vivo and thereby inhibits its ability to initiate SV40 DNA replication. The inhibition of both origin unwinding and DNA replication by the kinase is reversed by protein phosphatase 2A. As determined by molecular weight, substrate specificity, autophosphorylation, immunoreactivity, and limited sequence analysis, this kinase appears to be identical to casein kinase I, a ubiquitous serine/threonine protein kinase that is closely related to a yeast kinase involved in DNA metabolism. The HeLa cell phosphorylation cycle that controls the initiation of SV40 DNA replication may also play a role in cellular DNA metabolism.

Full Text

Duke Authors

Cited Authors

  • Cegielska, A; Virshup, DM

Published Date

  • February 1993

Published In

Volume / Issue

  • 13 / 2

Start / End Page

  • 1202 - 1211

PubMed ID

  • 8380893

Pubmed Central ID

  • PMC359005

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.13.2.1202-1211.1993


  • eng

Conference Location

  • United States