Human casein kinase Idelta phosphorylation of human circadian clock proteins period 1 and 2.

Published

Journal Article

Casein kinase Iepsilon (CKIepsilon), a central component of the circadian clock, interacts with and phosphorylates human period protein 1 (hPER1) [Keesler, G.A. et al. (2000) NeuroReport 5, 951-955]. A mutation in CKIepsilon causes a shortened circadian period in Syrian Golden hamster. We have now extended our previous studies to show that human casein kinase Idelta (hCKIdelta), the closest homologue to hCKIepsilon, associates with and phosphorylates hPER1 and causes protein instability. Furthermore, we observed that both hCKIdelta and hCKIepsilon phosphorylated and caused protein instability of human period 2 protein (hPER2). Immunohistochemical staining of rat brains demonstrates that CKIdelta protein is localized in the suprachiasmatic nuclei, the central location of the master clock. These results indicate that CKIdelta may play a role similar to CKIepsilon, suggesting that it may also be involved in regulating circadian rhythmicity by post-translation modification of mammalian clock proteins hPER1 and 2.

Full Text

Duke Authors

Cited Authors

  • Camacho, F; Cilio, M; Guo, Y; Virshup, DM; Patel, K; Khorkova, O; Styren, S; Morse, B; Yao, Z; Keesler, GA

Published Date

  • February 2, 2001

Published In

Volume / Issue

  • 489 / 2-3

Start / End Page

  • 159 - 165

PubMed ID

  • 11165242

Pubmed Central ID

  • 11165242

International Standard Serial Number (ISSN)

  • 0014-5793

Digital Object Identifier (DOI)

  • 10.1016/s0014-5793(00)02434-0

Language

  • eng

Conference Location

  • England