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Methylene blue inhibits neurogenic cholinergic vasodilator responses in the pulmonary vascular bed of the cat.

Publication ,  Journal Article
McMahon, TJ; Kadowitz, PJ
Published in: Am J Physiol
November 1992

The effects of methylene blue, an inhibitor of soluble guanylate cyclase, on pulmonary vasodilator responses to efferent vagal stimulation were investigated in the intact-chest cat under conditions of controlled blood flow and constant left atrial pressure. In animals pretreated with reserpine or phenoxybenzamine, under elevated tone conditions, efferent vagal stimulation at frequencies of 2-16 Hz caused stimulus-frequency-dependent decreases in lobar arterial pressure and pulmonary lobar vascular resistance. The vasodilator response to vagal stimulation was reproducible, blocked by atropine, and reduced by methylene blue. Intralobar infusion of methylene blue increased lobar arterial pressure without significantly altering systemic arterial or left atrial pressure. Methylene blue had no significant effect on vasodilator responses to isoproterenol, albuterol, atriopeptin III, lemakalim, adenosine, ATP, and pituitary adenylate cyclase-activating polypeptide-27 but significantly decreased vasodilator responses to acetylcholine, nitric oxide (NO), sodium nitroprusside, and the S-nitrosothiol, S-nitroso-N-acetyl-penicillamine. The effects of methylene blue on responses to vagal stimulation were reversible and were similar with the addition of a NO synthase inhibitor. The present data suggest that vasodilator responses to cholinergic nerve stimulation involve an increase in the production of guanosine 3',5'-cyclic monophosphate in the pulmonary vascular bed. These results provide additional evidence to support the hypothesis that neurogenically released acetylcholine induces endothelium-dependent, muscarinic, guanylate cyclase-mediated vasodilation.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

November 1992

Volume

263

Issue

5 Pt 1

Start / End Page

L575 / L584

Location

United States

Related Subject Headings

  • Vasodilation
  • Vasoconstrictor Agents
  • Vagus Nerve
  • Pulmonary Circulation
  • Prostaglandin Endoperoxides, Synthetic
  • Parasympathetic Nervous System
  • Nitric Oxide
  • NG-Nitroarginine Methyl Ester
  • Methylene Blue
  • Electric Stimulation
 

Citation

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MLA
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McMahon, T. J., & Kadowitz, P. J. (1992). Methylene blue inhibits neurogenic cholinergic vasodilator responses in the pulmonary vascular bed of the cat. Am J Physiol, 263(5 Pt 1), L575–L584. https://doi.org/10.1152/ajplung.1992.263.5.L575
McMahon, T. J., and P. J. Kadowitz. “Methylene blue inhibits neurogenic cholinergic vasodilator responses in the pulmonary vascular bed of the cat.Am J Physiol 263, no. 5 Pt 1 (November 1992): L575–84. https://doi.org/10.1152/ajplung.1992.263.5.L575.
McMahon, T. J., and P. J. Kadowitz. “Methylene blue inhibits neurogenic cholinergic vasodilator responses in the pulmonary vascular bed of the cat.Am J Physiol, vol. 263, no. 5 Pt 1, Nov. 1992, pp. L575–84. Pubmed, doi:10.1152/ajplung.1992.263.5.L575.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

November 1992

Volume

263

Issue

5 Pt 1

Start / End Page

L575 / L584

Location

United States

Related Subject Headings

  • Vasodilation
  • Vasoconstrictor Agents
  • Vagus Nerve
  • Pulmonary Circulation
  • Prostaglandin Endoperoxides, Synthetic
  • Parasympathetic Nervous System
  • Nitric Oxide
  • NG-Nitroarginine Methyl Ester
  • Methylene Blue
  • Electric Stimulation