Periods of systemic partial hypoxia induces apoptosis and inflammation in rat skeletal muscle.


Journal Article

Critical illness myopathy (CIM) causes significant morbidity. In this study, we investigated the effect of repeated mild hypoxia on the skeletal muscle inflammation. Sprague-Dawley rats anesthetized with 2% inhaled isoflurane were divided into two groups (n = 6 each), normoxia and hypoxia (12.5% for 12 min followed by 35% for 12 min, at which point the cycle was repeated for three times). We measured the tissue oxygen tension and perfusion (simultaneously) in hind limb skeletal muscle. Inflammation in skeletal muscle was assessed by light microcopy (Hematoxylin-Eosin staining) and apoptosis (Fluorescein-FragEL DNA fragmentation detection) and expressed as percent normoxia. Compared to the control group, hypoxia significantly (P < 0.001) altered histomorphometrics. Similarly, DNA fragmentation analysis revealed that hypoxia significantly (P < 0.001) induced apoptosis. We conclude that after a mild but repeated hypoxic insult there is marked histological alterations and induced apoptosis in skeletal muscle. We postulate that variable periods of hypoxia in the critically ill may be playing a role in the etiology of CIM.

Full Text

Cited Authors

  • Aravindan, N; Aravindan, S; Shanmugasundaram, K; Shaw, AD

Published Date

  • August 2007

Published In

Volume / Issue

  • 302 / 1-2

Start / End Page

  • 51 - 58

PubMed ID

  • 17323003

Pubmed Central ID

  • 17323003

International Standard Serial Number (ISSN)

  • 0300-8177

Digital Object Identifier (DOI)

  • 10.1007/s11010-007-9424-7


  • eng

Conference Location

  • Netherlands