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Neuron specific alpha-adrenergic receptor expression in human cerebellum: implications for emerging cerebellar roles in neurologic disease.

Publication ,  Journal Article
Schambra, UB; Mackensen, GB; Stafford-Smith, M; Haines, DE; Schwinn, DA
Published in: Neuroscience
2005

Recent data suggest novel functional roles for cerebellar involvement in a number of neurologic diseases. Function of cerebellar neurons is known to be modulated by norepinephrine and adrenergic receptors. The distribution of adrenergic receptor subtypes has been described in experimental animals, but corroboration of such studies in the human cerebellum, necessary for drug treatment, is still lacking. In the present work we studied cell-specific localizations of alpha1 adrenergic receptor subtype mRNA (alpha 1a, alpha 1b, alpha 1d), and alpha2 adrenergic receptor subtype mRNA (alpha 2a, alpha 2b, alpha 2c) by in situ hybridization on cryostat sections of human cerebellum (cortical layers and dentate nucleus). We observed unique neuron-specific alpha1 adrenergic receptor and alpha2 adrenergic receptor subtype distribution in human cerebellum. The cerebellar cortex expresses mRNA encoding all six alpha adrenergic receptor subtypes, whereas dentate nucleus neurons express all subtype mRNAs, except alpha 2a adrenergic receptor mRNA. All Purkinje cells label strongly for alpha 2a and alpha 2b adrenergic receptor mRNA. Additionally, Purkinje cells of the anterior lobe vermis (lobules I to V) and uvula/tonsil (lobules IX/HIX) express alpha 1a and alpha 2c subtypes, and Purkinje cells in the ansiform lobule (lobule HVII) and uvula/tonsil express alpha 1b and alpha 2c adrenergic receptor subtypes. Basket cells show a strong signal for alpha 1a, moderate signal for alpha 2a and light label for alpha 2b adrenergic receptor mRNA. In stellate cells, besides a strong label of alpha 2a adrenergic receptor mRNA in all and moderate label of alpha 2b message in select stellate cells, the inner stellate cells are also moderately positive for alpha 1b adrenergic receptor mRNA. Granule and Golgi cells express high levels of alpha 2a and alpha 2b adrenergic receptor mRNAs. These data contribute new information regarding specific location of adrenergic receptor subtypes in human cerebellar neurons. We discuss our observations in terms of possible modulatory roles of adrenergic receptor subtypes in cerebellar neurons responding to sensory and autonomic input signals, and review species differences in cerebellar adrenergic receptor expression.

Duke Scholars

Published In

Neuroscience

DOI

ISSN

0306-4522

Publication Date

2005

Volume

135

Issue

2

Start / End Page

507 / 523

Location

United States

Related Subject Headings

  • Receptors, Adrenergic, alpha
  • RNA, Messenger
  • Protein Subunits
  • Postmortem Changes
  • Neurons
  • Neurology & Neurosurgery
  • Nervous System Diseases
  • In Situ Hybridization
  • Humans
  • Gene Expression
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Schambra, U. B., Mackensen, G. B., Stafford-Smith, M., Haines, D. E., & Schwinn, D. A. (2005). Neuron specific alpha-adrenergic receptor expression in human cerebellum: implications for emerging cerebellar roles in neurologic disease. Neuroscience, 135(2), 507–523. https://doi.org/10.1016/j.neuroscience.2005.06.021
Schambra, U. B., G. B. Mackensen, M. Stafford-Smith, D. E. Haines, and D. A. Schwinn. “Neuron specific alpha-adrenergic receptor expression in human cerebellum: implications for emerging cerebellar roles in neurologic disease.Neuroscience 135, no. 2 (2005): 507–23. https://doi.org/10.1016/j.neuroscience.2005.06.021.
Schambra UB, Mackensen GB, Stafford-Smith M, Haines DE, Schwinn DA. Neuron specific alpha-adrenergic receptor expression in human cerebellum: implications for emerging cerebellar roles in neurologic disease. Neuroscience. 2005;135(2):507–23.
Schambra, U. B., et al. “Neuron specific alpha-adrenergic receptor expression in human cerebellum: implications for emerging cerebellar roles in neurologic disease.Neuroscience, vol. 135, no. 2, 2005, pp. 507–23. Pubmed, doi:10.1016/j.neuroscience.2005.06.021.
Schambra UB, Mackensen GB, Stafford-Smith M, Haines DE, Schwinn DA. Neuron specific alpha-adrenergic receptor expression in human cerebellum: implications for emerging cerebellar roles in neurologic disease. Neuroscience. 2005;135(2):507–523.
Journal cover image

Published In

Neuroscience

DOI

ISSN

0306-4522

Publication Date

2005

Volume

135

Issue

2

Start / End Page

507 / 523

Location

United States

Related Subject Headings

  • Receptors, Adrenergic, alpha
  • RNA, Messenger
  • Protein Subunits
  • Postmortem Changes
  • Neurons
  • Neurology & Neurosurgery
  • Nervous System Diseases
  • In Situ Hybridization
  • Humans
  • Gene Expression