Multiple alpha subunits of guanine nucleotide-binding proteins in Dictyostelium.

Journal Article

Previous results have shown that chemotaxis and the expression of several classes of genes in Dictyostelium discoideum are regulated through a cell surface cAMP receptor interacting with guanine nucleotide-binding proteins (G proteins). We now describe cloning and sequencing of cDNAs encoding two G alpha protein subunits from Dictyostelium. The derived amino acid sequences show that they are 45% identical to each other and to G alpha protein subunits from mammals and yeast. Both cDNAs are complementary to multiple mRNAs that are differentially expressed during development. This evidence and analysis of mutants presented elsewhere suggest that they have distinct physiological functions.

Full Text

Duke Authors

Cited Authors

  • Pupillo, M; Kumagai, A; Pitt, GS; Firtel, RA; Devreotes, PN

Published Date

  • July 1, 1989

Published In

Volume / Issue

  • 86 / 13

Start / End Page

  • 4892 - 4896

PubMed ID

  • 2500658

International Standard Serial Number (ISSN)

  • 0027-8424

Language

  • eng

Conference Location

  • United States