Adenylyl cyclase G, an osmosensor controlling germination of Dictyostelium spores.

Published

Journal Article

Dictyostelium cells express a G-protein-coupled adenylyl cyclase, ACA, during aggregation and an atypical adenylyl cyclase, ACG, in mature spores. The ACG gene was disrupted by homologous recombination. acg- cells developed into normal fruiting bodies with viable spores, but spore germination was no longer inhibited by high osmolarity, a fairly universal constraint for spore and seed germination. ACG activity, measured in aca-/ACG cells, was strongly stimulated by high osmolarity with optimal stimulation occurring at 200 milliosmolar. RdeC mutants, which display unrestrained protein kinase A (PKA) activity and a cell line, which overexpresses PKA under a prespore specific promoter, germinate very poorly, both at high and low osmolarity. These data indicate that ACG is an osmosensor controlling spore germination through activation of protein kinase A.

Full Text

Duke Authors

Cited Authors

  • van Es, S; Virdy, KJ; Pitt, GS; Meima, M; Sands, TW; Devreotes, PN; Cotter, DA; Schaap, P

Published Date

  • September 27, 1996

Published In

Volume / Issue

  • 271 / 39

Start / End Page

  • 23623 - 23625

PubMed ID

  • 8798577

Pubmed Central ID

  • 8798577

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.271.39.23623

Language

  • eng

Conference Location

  • United States