Defective T cell development and function in the absence of Abelson kinases.

Journal Article

Thymocyte proliferation, survival, and differentiation are tightly controlled by signaling from the pre-TCR. In this study, we show for the first time that the Abelson (Abl) kinases regulate proximal signaling downstream of the pre-TCR. Conditional deletion of Abl kinases in thymocytes reveals a cell-autonomous role for these proteins in T cell development. The conditional knockout mice have reduced numbers of thymocytes, exhibit an increase in the percentage of the CD4(-)CD8(-) double-negative population, and are partially blocked in the transition to the CD4(+)CD8(+) double-positive stage. Moreover, the total number of T cells is greatly reduced in the Abl mutant mice, and the null T cells exhibit impaired TCR-induced signaling, proliferation, and cytokine production. Notably, Abl mutant mice are compromised in their ability to produce IFN-positive CD8 T cells and exhibit impaired CD8(+) T cell expansion in vivo upon Listeria monocytogenes infection. Furthermore, Ab production in response to T cell-dependent Ag is severely impaired in the Abl mutant mice. Together these findings reveal cell-autonomous roles for the Abl family kinases in both T cell development and mature T cell function, and show that loss of these kinases specifically in T cells results in compromised immunity.

Full Text

Duke Authors

Cited Authors

  • Gu, JJ; Zhang, N; He, Y-W; Koleske, AJ; Pendergast, AM

Published Date

  • December 1, 2007

Published In

Volume / Issue

  • 179 / 11

Start / End Page

  • 7334 - 7343

PubMed ID

  • 18025176

International Standard Serial Number (ISSN)

  • 0022-1767

Language

  • eng

Conference Location

  • United States