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Hyperbaric oxygen reduces cerebral blood flow by inactivating nitric oxide.

Publication ,  Journal Article
Demchenko, IT; Boso, AE; Bennett, PB; Whorton, AR; Piantadosi, CA
Published in: Nitric Oxide
December 2000

Based on recent evidence that nitric oxide (NO(.)) is involved in hyperoxic vasoconstriction, we tested the hypothesis that decreases in NO(.) availability in brain tissue during hyperbaric oxygen (HBO(2)) exposure contribute to decreases in regional cerebral blood flow (rCBF). rCBF was measured in rats exposed to HBO(2) at 5 atmospheres (ATA) and correlated with interstitial brain levels of NO(.) metabolites (NO(X)) and production of hydroxyl radical ((.)OH). Changes in rCBF were also correlated with the effects of NO(.) synthase inhibitor (l-NAME), NO(.) donor PAPANONOate, and intravascular superoxide dismutase (MnSOD) during HBO(2). After 30 min of O(2) exposure at 5 ATA, rCBF had decreased in the substantia nigra, caudate putamen, hippocampus, and parietal cortex by 23 to 37%. These reductions in rCBF were not augmented by exposure to HBO(2) in animals pre-treated with l-NAME. After 30 min at 5 ATA, brain NO(X) levels had decreased by 31 +/- 9% and correlated with the decrease in rCBF, while estimated (.)OH production increased by 56 +/- 8%. The decrease in rCBF at 5 ATA was completely abolished by MnSOD administration into the circulation before HBO(2) exposure. Doses of NO(.) donor that significantly increased rCBF in animals breathing air had no effect at 5 ATA of HBO(2). These results indicate that decreases in rCBF with HBO(2) are associated with a decrease in effective NO(.) concentration and an increase in ROS production in the brain. The data support the hypothesis that inactivation of NO(.) antagonizes basal relaxation of cerebral vessels during HBO(2) exposure, although an effect of HBO(2) on NO(.) synthesis has not been excluded.

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Published In

Nitric Oxide

DOI

ISSN

1089-8603

Publication Date

December 2000

Volume

4

Issue

6

Start / End Page

597 / 608

Location

United States

Related Subject Headings

  • Superoxide Dismutase
  • Rats, Sprague-Dawley
  • Rats
  • Nitrites
  • Nitric Oxide
  • Nitrates
  • NG-Nitroarginine Methyl Ester
  • Microdialysis
  • Injections, Intravenous
  • Hyperbaric Oxygenation
 

Citation

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Demchenko, I. T., Boso, A. E., Bennett, P. B., Whorton, A. R., & Piantadosi, C. A. (2000). Hyperbaric oxygen reduces cerebral blood flow by inactivating nitric oxide. Nitric Oxide, 4(6), 597–608. https://doi.org/10.1006/niox.2000.0313
Demchenko, I. T., A. E. Boso, P. B. Bennett, A. R. Whorton, and C. A. Piantadosi. “Hyperbaric oxygen reduces cerebral blood flow by inactivating nitric oxide.Nitric Oxide 4, no. 6 (December 2000): 597–608. https://doi.org/10.1006/niox.2000.0313.
Demchenko IT, Boso AE, Bennett PB, Whorton AR, Piantadosi CA. Hyperbaric oxygen reduces cerebral blood flow by inactivating nitric oxide. Nitric Oxide. 2000 Dec;4(6):597–608.
Demchenko, I. T., et al. “Hyperbaric oxygen reduces cerebral blood flow by inactivating nitric oxide.Nitric Oxide, vol. 4, no. 6, Dec. 2000, pp. 597–608. Pubmed, doi:10.1006/niox.2000.0313.
Demchenko IT, Boso AE, Bennett PB, Whorton AR, Piantadosi CA. Hyperbaric oxygen reduces cerebral blood flow by inactivating nitric oxide. Nitric Oxide. 2000 Dec;4(6):597–608.
Journal cover image

Published In

Nitric Oxide

DOI

ISSN

1089-8603

Publication Date

December 2000

Volume

4

Issue

6

Start / End Page

597 / 608

Location

United States

Related Subject Headings

  • Superoxide Dismutase
  • Rats, Sprague-Dawley
  • Rats
  • Nitrites
  • Nitric Oxide
  • Nitrates
  • NG-Nitroarginine Methyl Ester
  • Microdialysis
  • Injections, Intravenous
  • Hyperbaric Oxygenation