Determination of immunoreactive endothelin in medium from cultured endothelial cells and human plasma.

Published

Journal Article

We have developed a sensitive and selective radioimmunoassay for porcine/human endothelin (ET1). The assay has a detection limit of 0.62 pg/tube and exhibits no cross-reactivity to atrial natriuretic peptide, arginine vasopressin, or angiotensin II. Procedures were developed for extraction of endothelin from human plasma samples and samples of buffer from endothelial cell incubations using C18 Sep-Pak extraction cartridges. The mean recovery following extraction was approximately 80%. Both bovine and porcine aortic endothelial cells were found to produce immunoreactive endothelin (IR-ET) with porcine cells producing 4.7 +/- 1.1 ng of IR-ET/mg cell protein after 6 hours. Human plasma samples were extracted, assayed and found to contain a mean concentration of 2.0 +/- 0.4 pg/ml of IR-ET.

Full Text

Cited Authors

  • Xuan, YT; Whorton, AR; Shearer-Poor, E; Boyd, J; Watkins, WD

Published Date

  • October 1989

Published In

Volume / Issue

  • 164 / 1

Start / End Page

  • 326 - 332

PubMed ID

  • 2679553

Pubmed Central ID

  • 2679553

Electronic International Standard Serial Number (EISSN)

  • 1090-2104

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/0006-291x(89)91721-x

Language

  • eng