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A genetically tractable model of human glioma formation.

Publication ,  Journal Article
Rich, JN; Guo, C; McLendon, RE; Bigner, DD; Wang, XF; Counter, CM
Published in: Cancer Res
May 1, 2001

Gliomas remain one of the deadliest forms of cancer. Improved therapeutics will require a better understanding of the molecular nature of these tumors. We, therefore, mimicked the most common genetic changes found in grade III-IV gliomas, disruption of the p53 and RB pathways and activation of telomere maintenance and independence from growth factors, through the ectopic expression of the SV40 T/t-Ag oncogene, an oncogenic form of H-ras (H-ras(V12G)), and the human telomerase catalytic subunit hTERT in normal human astrocytes. The resulting cells displayed many of the hallmarks of grade III-IV gliomas, including greatly expanded life span and growth in soft agar and, most importantly, were tumorigenic with pathology consistent with grade III-IV neuroectodermal tumors in mice. This model system will, for the first time, allow the biological significance of selected genetic alterations to be studied in human gliomas.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

May 1, 2001

Volume

61

Issue

9

Start / End Page

3556 / 3560

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Telomerase
  • Retroviridae
  • RNA
  • Oncology & Carcinogenesis
  • Mice, SCID
  • Mice, Nude
  • Mice, Inbred BALB C
  • Mice
  • Humans
 

Citation

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ICMJE
MLA
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Rich, J. N., Guo, C., McLendon, R. E., Bigner, D. D., Wang, X. F., & Counter, C. M. (2001). A genetically tractable model of human glioma formation. Cancer Res, 61(9), 3556–3560.
Rich, J. N., C. Guo, R. E. McLendon, D. D. Bigner, X. F. Wang, and C. M. Counter. “A genetically tractable model of human glioma formation.Cancer Res 61, no. 9 (May 1, 2001): 3556–60.
Rich JN, Guo C, McLendon RE, Bigner DD, Wang XF, Counter CM. A genetically tractable model of human glioma formation. Cancer Res. 2001 May 1;61(9):3556–60.
Rich, J. N., et al. “A genetically tractable model of human glioma formation.Cancer Res, vol. 61, no. 9, May 2001, pp. 3556–60.
Rich JN, Guo C, McLendon RE, Bigner DD, Wang XF, Counter CM. A genetically tractable model of human glioma formation. Cancer Res. 2001 May 1;61(9):3556–3560.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

May 1, 2001

Volume

61

Issue

9

Start / End Page

3556 / 3560

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Telomerase
  • Retroviridae
  • RNA
  • Oncology & Carcinogenesis
  • Mice, SCID
  • Mice, Nude
  • Mice, Inbred BALB C
  • Mice
  • Humans