Glioma-associated antigen expression in oligodendroglial neoplasms. Tenascin and epidermal growth factor receptor.

Published

Journal Article

Epidermal growth factor receptor (EGFR), its variant, EGFRvIII, and tenascin are glioma-associated antigens that are hyperexpressed by neoplastic glial cells relative to normal brain, making them attractive antigenic targets for immunotherapy. Preliminary surveys indicate that oligodendroglial tumors also produce these proteins, although the exact patterns and degrees of reactivity are not known. In this study we examined the immunoreactivity of tenascin among 50 oligodendroglial tumors, including 25 well-differentiated oligodendrogliomas (WDOs) and 12 glioblastomas (GBMs) exhibiting high proportions of oligodendroglia-like cells. We used well-characterized immunoreagents with defined specificities against the target antigens on formalin-fixed, paraffin-embedded archival tissue. The tumors were graded according to WHO guidelines. Immunoreactivity was reported on a 1-3 scale according to staining intensity multiplied by a 1-3 distribution scale distribution within tumor as focal (1), multifocal (2), and diffuse (3) for both the parenchymal and the perivascular components. Although there is considerable overlap in antigen production among the grades of tumor, this study establishes the production of tenascin and wild-type EGFR (but not EGFR vIII) in oligodendroglial neoplasms and supports the concept that antigen production increases with tumor grade.

Full Text

Duke Authors

Cited Authors

  • McLendon, RE; Wikstrand, CJ; Matthews, MR; Al-Baradei, R; Bigner, SH; Bigner, DD

Published Date

  • August 2000

Published In

Volume / Issue

  • 48 / 8

Start / End Page

  • 1103 - 1110

PubMed ID

  • 10898803

Pubmed Central ID

  • 10898803

International Standard Serial Number (ISSN)

  • 0022-1554

Digital Object Identifier (DOI)

  • 10.1177/002215540004800807

Language

  • eng

Conference Location

  • United States