Genome mapping by fluorescent fingerprinting.

Published

Journal Article

The construction of sequence-ready maps of overlapping genomic clones is central to large-scale genome sequencing. We have implemented a method for fluorescent fingerprinting of bacterial clones to assemble contig maps. The method utilizes three spectrally distinct fluorescently tagged dideoxy ATPs to specifically label the HindIII termini in HindIII and Sau3AI restriction digests of clones that are multiplexed prior to electrophoresis and data collection. There is excellent reproducibility of raw data, improved resolution of large fragments, and concordance between the results obtained using this and the equivalent radioactive protocol. This method also allows detection of smaller overlaps between clones when compared to the analysis of restriction digests on nondenaturing agarose gels.

Full Text

Duke Authors

Cited Authors

  • Gregory, SG; Howell, GR; Bentley, DR

Published Date

  • December 1997

Published In

Volume / Issue

  • 7 / 12

Start / End Page

  • 1162 - 1168

PubMed ID

  • 9414321

Pubmed Central ID

  • 9414321

International Standard Serial Number (ISSN)

  • 1088-9051

Digital Object Identifier (DOI)

  • 10.1101/gr.7.12.1162

Language

  • eng

Conference Location

  • United States