Enhancing linkage analysis of complex disorders: an evaluation of high-density genotyping.

Journal Article (Journal Article)

To explore the potential value of recently developed high-density linkage mapping methods in the analysis of complex disease we have regenotyped five nuclear families first studied in the 1996 UK multiple sclerosis linkage genome screen, using Applied Biosystems high-density microsatellite linkage mapping set, the Illumina BeadArray linkage mapping panel (version 3) and the Affymetrix GeneChip Human Mapping 10K array. We found that genotyping success, information extraction and genotyping accuracy were improved with all systems. These improvements were particularly marked with the SNP-based methods (Illumina and Affymetrix), with little difference between these. The extent of additional information extracted is considerable, indicating that reanalysis of existing multiplex families using these newer systems would substantially increase power.

Full Text

Duke Authors

Cited Authors

  • Sawcer, SJ; Maranian, M; Singlehurst, S; Yeo, T; Compston, A; Daly, MJ; De Jager, PL; Gabriel, S; Hafler, DA; Ivinson, AJ; Lander, ES; Rioux, JD; Walsh, E; Gregory, SG; Schmidt, S; Pericak-Vance, MA; Barcellos, L; Hauser, SL; Oksenberg, JR; Kenealy, SJ; Haines, JL

Published Date

  • September 1, 2004

Published In

Volume / Issue

  • 13 / 17

Start / End Page

  • 1943 - 1949

PubMed ID

  • 15238506

International Standard Serial Number (ISSN)

  • 0964-6906

Digital Object Identifier (DOI)

  • 10.1093/hmg/ddh202


  • eng

Conference Location

  • England