Multiple roles for the active zone protein RIM1alpha in late stages of neurotransmitter release.

Published

Journal Article

The active zone protein RIM1alpha interacts with multiple active zone and synaptic vesicle proteins and is implicated in short- and long-term synaptic plasticity, but it is unclear how RIM1alpha's biochemical interactions translate into physiological functions. To address this question, we analyzed synaptic transmission in autaptic neurons cultured from RIM1alpha-/- mice. Deletion of RIM1alpha causes a large reduction in the readily releasable pool of vesicles, alters short-term plasticity, and changes the properties of evoked asynchronous release. Lack of RIM1alpha, however, had no effect on synapse formation, spontaneous release, overall Ca2+ sensitivity of release, or synaptic vesicle recycling. These results suggest that RIM1alpha modulates sequential steps in synaptic vesicle exocytosis through serial protein-protein interactions and that this modulation is the basis for RIM1alpha's role in synaptic plasticity.

Full Text

Duke Authors

Cited Authors

  • Calakos, N; Schoch, S; Südhof, TC; Malenka, RC

Published Date

  • June 24, 2004

Published In

Volume / Issue

  • 42 / 6

Start / End Page

  • 889 - 896

PubMed ID

  • 15207234

Pubmed Central ID

  • 15207234

International Standard Serial Number (ISSN)

  • 0896-6273

Digital Object Identifier (DOI)

  • 10.1016/j.neuron.2004.05.014

Language

  • eng

Conference Location

  • United States