The study of protein mechanics with the atomic force microscope.

Journal Article (Journal Article)

The unfolding and folding of single protein molecules can be studied with an atomic force microscope (AFM). Many proteins with mechanical functions contain multiple, individually folded domains with similar structures. Protein engineering techniques have enabled the construction and expression of recombinant proteins that contain multiple copies of identical domains. Thus, the AFM in combination with protein engineering has enabled the kinetic analysis of the force-induced unfolding and refolding of individual domains as well as the study of the determinants of mechanical stability.

Full Text

Duke Authors

Cited Authors

  • Fisher, TE; Oberhauser, AF; Carrion-Vazquez, M; Marszalek, PE; Fernandez, JM

Published Date

  • October 1999

Published In

Volume / Issue

  • 24 / 10

Start / End Page

  • 379 - 384

PubMed ID

  • 10500301

International Standard Serial Number (ISSN)

  • 0968-0004

Digital Object Identifier (DOI)

  • 10.1016/s0968-0004(99)01453-x


  • eng