Single protein misfolding events captured by atomic force microscopy.

Journal Article (Journal Article)

Using single protein atomic force microscopy (AFM) techniques we demonstrate that after repeated mechanical extension/relaxation cycles, tandem modular proteins can misfold into a structure formed by two neighboring modules. The misfolding is fully reversible and alters the mechanical topology of the modules while it is about as stable as the original fold. Our results show that modular proteins can assume a novel misfolded state and demonstrate that AFM is able to capture, in real time, rare misfolding events at the level of a single protein.

Full Text

Duke Authors

Cited Authors

  • Oberhauser, AF; Marszalek, PE; Carrion-Vazquez, M; Fernandez, JM

Published Date

  • November 1999

Published In

Volume / Issue

  • 6 / 11

Start / End Page

  • 1025 - 1028

PubMed ID

  • 10542093

International Standard Serial Number (ISSN)

  • 1072-8368

Digital Object Identifier (DOI)

  • 10.1038/14907


  • eng