Sound waves are excited in an optically trapped degenerate Fermi gas of spin-up and spin-down atoms with magnetically tunable interactions. Measurements are made throughout the crossover region, from a weakly interacting Fermi gas through the resonant Fermi superfluid regime to a Bose condensate of dimer molecules. The measured sound velocities test theories of hydrodynamic wave propagation and predictions of the equation of state. At resonance, the sound velocity exhibits universal scaling with the Fermi velocity, to within 1.8% over a factor of 30 in density. © 2007 The American Physical Society.