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Human granulosa-luteal and cumulus cells express transforming growth factors-beta type 1 and type 2 mRNA.

Publication ,  Journal Article
Mulheron, GW; Bossert, NL; Lapp, JA; Walmer, DK; Schomberg, DW
Published in: J Clin Endocrinol Metab
February 1992

Human granulosa-luteal cells and cumulus cells obtained from women undergoing in vitro fertilization and embryo transfer (IVF-ET) were examined for the presence of TGF-beta 1 and TGF-beta 2 mRNA by reverse transcription-polymerase chain reaction (RT-PCR) analysis. RT-PCR analysis revealed that both follicle cell types express mRNA for both TGF-beta subtypes. Verification of RT-PCR products was done by restriction enzyme digestion analysis. These results suggest a role(s) for TGF-beta 1 and TGF-beta 2 in the development of human granulosa-luteal cells and the oocyte-cumulus cell complex.

Duke Scholars

Published In

J Clin Endocrinol Metab

DOI

ISSN

0021-972X

Publication Date

February 1992

Volume

74

Issue

2

Start / End Page

458 / 460

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta
  • Superovulation
  • RNA, Messenger
  • RNA
  • Polymerase Chain Reaction
  • Ovary
  • Oligodeoxyribonucleotides
  • Molecular Sequence Data
  • Menotropins
  • Humans
 

Citation

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Mulheron, G. W., Bossert, N. L., Lapp, J. A., Walmer, D. K., & Schomberg, D. W. (1992). Human granulosa-luteal and cumulus cells express transforming growth factors-beta type 1 and type 2 mRNA. J Clin Endocrinol Metab, 74(2), 458–460. https://doi.org/10.1210/jcem.74.2.1370508
Mulheron, G. W., N. L. Bossert, J. A. Lapp, D. K. Walmer, and D. W. Schomberg. “Human granulosa-luteal and cumulus cells express transforming growth factors-beta type 1 and type 2 mRNA.J Clin Endocrinol Metab 74, no. 2 (February 1992): 458–60. https://doi.org/10.1210/jcem.74.2.1370508.
Mulheron GW, Bossert NL, Lapp JA, Walmer DK, Schomberg DW. Human granulosa-luteal and cumulus cells express transforming growth factors-beta type 1 and type 2 mRNA. J Clin Endocrinol Metab. 1992 Feb;74(2):458–60.
Mulheron, G. W., et al. “Human granulosa-luteal and cumulus cells express transforming growth factors-beta type 1 and type 2 mRNA.J Clin Endocrinol Metab, vol. 74, no. 2, Feb. 1992, pp. 458–60. Pubmed, doi:10.1210/jcem.74.2.1370508.
Mulheron GW, Bossert NL, Lapp JA, Walmer DK, Schomberg DW. Human granulosa-luteal and cumulus cells express transforming growth factors-beta type 1 and type 2 mRNA. J Clin Endocrinol Metab. 1992 Feb;74(2):458–460.
Journal cover image

Published In

J Clin Endocrinol Metab

DOI

ISSN

0021-972X

Publication Date

February 1992

Volume

74

Issue

2

Start / End Page

458 / 460

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta
  • Superovulation
  • RNA, Messenger
  • RNA
  • Polymerase Chain Reaction
  • Ovary
  • Oligodeoxyribonucleotides
  • Molecular Sequence Data
  • Menotropins
  • Humans