Human granulosa-luteal and cumulus cells express transforming growth factors-beta type 1 and type 2 mRNA.
Publication
, Journal Article
Mulheron, GW; Bossert, NL; Lapp, JA; Walmer, DK; Schomberg, DW
Published in: J Clin Endocrinol Metab
February 1992
Human granulosa-luteal cells and cumulus cells obtained from women undergoing in vitro fertilization and embryo transfer (IVF-ET) were examined for the presence of TGF-beta 1 and TGF-beta 2 mRNA by reverse transcription-polymerase chain reaction (RT-PCR) analysis. RT-PCR analysis revealed that both follicle cell types express mRNA for both TGF-beta subtypes. Verification of RT-PCR products was done by restriction enzyme digestion analysis. These results suggest a role(s) for TGF-beta 1 and TGF-beta 2 in the development of human granulosa-luteal cells and the oocyte-cumulus cell complex.
Duke Scholars
Published In
J Clin Endocrinol Metab
DOI
ISSN
0021-972X
Publication Date
February 1992
Volume
74
Issue
2
Start / End Page
458 / 460
Location
United States
Related Subject Headings
- Transforming Growth Factor beta
- Superovulation
- RNA, Messenger
- RNA
- Polymerase Chain Reaction
- Ovary
- Oligodeoxyribonucleotides
- Molecular Sequence Data
- Menotropins
- Humans
Citation
APA
Chicago
ICMJE
MLA
NLM
Mulheron, G. W., Bossert, N. L., Lapp, J. A., Walmer, D. K., & Schomberg, D. W. (1992). Human granulosa-luteal and cumulus cells express transforming growth factors-beta type 1 and type 2 mRNA. J Clin Endocrinol Metab, 74(2), 458–460. https://doi.org/10.1210/jcem.74.2.1370508
Mulheron, G. W., N. L. Bossert, J. A. Lapp, D. K. Walmer, and D. W. Schomberg. “Human granulosa-luteal and cumulus cells express transforming growth factors-beta type 1 and type 2 mRNA.” J Clin Endocrinol Metab 74, no. 2 (February 1992): 458–60. https://doi.org/10.1210/jcem.74.2.1370508.
Mulheron GW, Bossert NL, Lapp JA, Walmer DK, Schomberg DW. Human granulosa-luteal and cumulus cells express transforming growth factors-beta type 1 and type 2 mRNA. J Clin Endocrinol Metab. 1992 Feb;74(2):458–60.
Mulheron, G. W., et al. “Human granulosa-luteal and cumulus cells express transforming growth factors-beta type 1 and type 2 mRNA.” J Clin Endocrinol Metab, vol. 74, no. 2, Feb. 1992, pp. 458–60. Pubmed, doi:10.1210/jcem.74.2.1370508.
Mulheron GW, Bossert NL, Lapp JA, Walmer DK, Schomberg DW. Human granulosa-luteal and cumulus cells express transforming growth factors-beta type 1 and type 2 mRNA. J Clin Endocrinol Metab. 1992 Feb;74(2):458–460.
Published In
J Clin Endocrinol Metab
DOI
ISSN
0021-972X
Publication Date
February 1992
Volume
74
Issue
2
Start / End Page
458 / 460
Location
United States
Related Subject Headings
- Transforming Growth Factor beta
- Superovulation
- RNA, Messenger
- RNA
- Polymerase Chain Reaction
- Ovary
- Oligodeoxyribonucleotides
- Molecular Sequence Data
- Menotropins
- Humans