Amplification of HIV-1 provirus from cerebrospinal fluid and its correlation with neurologic disease.

Journal Article (Journal Article)

The polymerase chain reaction (PCR) was used to detect human immunodeficiency virus type 1 (HIV-1) proviral sequences (gag and env) in nucleated cells from the cerebrospinal fluid (CSF) of 31 HIV-1-positive patients, and the results were compared with clinical and radiologic evidence of neurologic disease. Provirus was detected in 21 patients, of whom 20 had neurologic abnormalities. Provirus was not detected in another 6, all of whom were neurologically normal. No neurologic disease has developed in 4 of these 6 patients for whom 12.8 months of follow-up is available. PCR of CSF nucleated cells from HIV-positive patients provides early, rapid, direct evidence of neurologic involvement.

Full Text

Duke Authors

Cited Authors

  • Shaunak, S; Albright, RE; Klotman, ME; Henry, SC; Bartlett, JA; Hamilton, JD

Published Date

  • June 1, 1990

Published In

Volume / Issue

  • 161 / 6

Start / End Page

  • 1068 - 1072

PubMed ID

  • 2189004

International Standard Serial Number (ISSN)

  • 0022-1899

Digital Object Identifier (DOI)

  • 10.1093/infdis/161.6.1068


  • eng

Conference Location

  • United States