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Enhanced green fluorescent protein as a marker for localizing murine cytomegalovirus in acute and latent infection.

Publication ,  Journal Article
Henry, SC; Schmader, K; Brown, TT; Miller, SE; Howell, DN; Daley, GG; Hamilton, JD
Published in: J Virol Methods
September 2000

A recombinant murine cytomegalovirus (mCMV) that expresses enhanced green fluorescent protein (EGFP) under control of the native immediate-early 1/3 promoter was constructed to detect directly sites of viral activity in latent and reactivated infections. The recombinant virus had acute and latent infection characteristics similar to those of wild-type mCMV. Rare green-fluorescing foci were observed in paraffin sections from lungs and spleens infected latently. Positive immunoperoxidase staining for EGFP in sections of the same lung tissues suggests that these cells may be sites of restricted viral gene expression. EGFP was detected easily in tissue explants reactivating from latent infection in vitro. Morphology and adhesion characteristics of fluorescing cells suggest that viral reactivation occurs in tissue macrophages in explant cultures. The observations presented in this study demonstrate the usefulness of EGFP-expressing recombinants as tools for direct tracking of mCMV activity in vivo and in vitro.

Duke Scholars

Published In

J Virol Methods

DOI

ISSN

0166-0934

Publication Date

September 2000

Volume

89

Issue

1-2

Start / End Page

61 / 73

Location

Netherlands

Related Subject Headings

  • Virus Latency
  • Virus Activation
  • Virology
  • Promoter Regions, Genetic
  • Muromegalovirus
  • Microscopy, Fluorescence
  • Microscopy, Confocal
  • Mice, Inbred BALB C
  • Mice
  • Luminescent Proteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Henry, S. C., Schmader, K., Brown, T. T., Miller, S. E., Howell, D. N., Daley, G. G., & Hamilton, J. D. (2000). Enhanced green fluorescent protein as a marker for localizing murine cytomegalovirus in acute and latent infection. J Virol Methods, 89(1–2), 61–73. https://doi.org/10.1016/s0166-0934(00)00202-0
Henry, S. C., K. Schmader, T. T. Brown, S. E. Miller, D. N. Howell, G. G. Daley, and J. D. Hamilton. “Enhanced green fluorescent protein as a marker for localizing murine cytomegalovirus in acute and latent infection.J Virol Methods 89, no. 1–2 (September 2000): 61–73. https://doi.org/10.1016/s0166-0934(00)00202-0.
Henry SC, Schmader K, Brown TT, Miller SE, Howell DN, Daley GG, et al. Enhanced green fluorescent protein as a marker for localizing murine cytomegalovirus in acute and latent infection. J Virol Methods. 2000 Sep;89(1–2):61–73.
Henry, S. C., et al. “Enhanced green fluorescent protein as a marker for localizing murine cytomegalovirus in acute and latent infection.J Virol Methods, vol. 89, no. 1–2, Sept. 2000, pp. 61–73. Pubmed, doi:10.1016/s0166-0934(00)00202-0.
Henry SC, Schmader K, Brown TT, Miller SE, Howell DN, Daley GG, Hamilton JD. Enhanced green fluorescent protein as a marker for localizing murine cytomegalovirus in acute and latent infection. J Virol Methods. 2000 Sep;89(1–2):61–73.
Journal cover image

Published In

J Virol Methods

DOI

ISSN

0166-0934

Publication Date

September 2000

Volume

89

Issue

1-2

Start / End Page

61 / 73

Location

Netherlands

Related Subject Headings

  • Virus Latency
  • Virus Activation
  • Virology
  • Promoter Regions, Genetic
  • Muromegalovirus
  • Microscopy, Fluorescence
  • Microscopy, Confocal
  • Mice, Inbred BALB C
  • Mice
  • Luminescent Proteins