Transmission of latent cytomegalovirus in a murine kidney tissue transplantation model.
This paper examines the hypothesis that latent murine cytomegalovirus (MCMV) may be transmitted in kidney tissue to transplant recipients. Balb/c mice were infected with MCMV, and at intervals of less than 1 week to greater than 1 year, transmission of the virus from infected donors was attempted by transplantation of kidney sections or transfusion of blood into uninfected recipients. Graft recipients were killed from 2-4 weeks later, and cultured for MCMV. Restriction endonuclease digestion profiles of viral DNA were performed. Acutely infected donors transmitted MCMV in kidney tissue to 83-66% of untreated, susceptible recipients. Latently infected donors transmitted the infecting strain of virus to 20% of all and 31% of immunosuppressed recipients but to 37% of the syngeneic versus 21% of the allogeneic (P less than .027). Blood transfusions transmitted latent virus to 28% of recipients. In conclusion, kidney tissue may serve as the source of latent virus in this murine transplantation model. Retained blood in the kidney is unlikely to be the only source of virus.
Hamilton, JD; Seaworth, BJ
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