Multi-step control of spindle pole body duplication by cyclin-dependent kinase.

Published

Journal Article

Organelles called centrosomes in metazoans or spindle pole bodies (SPBs) in yeast direct the assembly of a bipolar spindle that is essential for faithful segregation of chromosomes during mitosis. Abnormal accumulation of multiple centrosomes leads to genome instability, and has been observed in both tumour cells and cells with targeted mutations in tumour-suppressor genes. The defects that lead to centrosome amplification are not understood. We have recapitulated the multiple-centrosome phenotype in budding yeast by disrupting the activity of specific cyclin-dependent kinase (CDK) complexes. Our observations are reminiscent of mechanisms that govern DNA replication, and show that specific cyclin/CDK activities function both to promote SPB duplication and to prevent SPB reduplication.

Full Text

Duke Authors

Cited Authors

  • Haase, SB; Winey, M; Reed, SI

Published Date

  • January 2001

Published In

Volume / Issue

  • 3 / 1

Start / End Page

  • 38 - 42

PubMed ID

  • 11146624

Pubmed Central ID

  • 11146624

Electronic International Standard Serial Number (EISSN)

  • 1476-4679

International Standard Serial Number (ISSN)

  • 1465-7392

Digital Object Identifier (DOI)

  • 10.1038/35050543

Language

  • eng