Improved EBV shuttle vectors.

Published

Journal Article

Shuttle vectors based on Epstein-Barr virus (EBV) replicate autonomously in the nuclei of human cells. These vectors represent reasonable models for chromosomes, have low background mutation frequencies, and have been useful for studying induced mutation in human cells. Two improvements in the EBV vector system are discussed. Attempts are described to increase vector copy number per cell by using a limited period of replication driven by the simian virus 40 (SV40) origin of replication. Isolation of human sequences that can replace the viral origin of replication in providing for autonomous replication of the vectors is also described. These improvements are leading toward shuttle vectors that are more efficient and more closely resemble authentic chromosomes.

Full Text

Duke Authors

Cited Authors

  • Haase, SB; Heinzel, SS; Krysan, PJ; Calos, MP

Published Date

  • March 1989

Published In

Volume / Issue

  • 220 / 2-3

Start / End Page

  • 125 - 132

PubMed ID

  • 2538736

Pubmed Central ID

  • 2538736

Electronic International Standard Serial Number (EISSN)

  • 1873-135X

International Standard Serial Number (ISSN)

  • 0027-5107

Digital Object Identifier (DOI)

  • 10.1016/0165-1110(89)90018-3

Language

  • eng