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Age-related alterations in actin cytoskeleton and receptor expression in human leukocytes.

Publication ,  Journal Article
Rao, KM; Currie, MS; Padmanabhan, J; Cohen, HJ
Published in: J Gerontol
March 1992

We studied a number of parameters, which may all depend upon cytoskeletal function, comparing lymphocytes and granulocytes (PMN) from young and old healthy donors. F-actin content was measured by NBD-phallacidin staining, followed by flow cytometry and was expressed as mean channel fluorescence (MCF). There were no differences in the basal F-actin content of PMN obtained from young (under 35 years old) and old donors (above 65 years). In contrast, the basal F-actin content was higher in lymphocytes obtained from the old donors (MCF, 56.8 +/- 2.9 vs 48.1 +/- 2.6 in the young; mean +/- SEM, n = 20, p less than .03). Stimulus-induced actin polymerization was slightly lower in the older age-group both in PMN and lymphocytes, but a significant difference was found only in PMN stimulated with the chemotactic peptide, N-formyl-methionyl-leucyl-phenylalanine (MCF 97.9 +/- 4.7 vs 88.6 +/- 3.4; young vs old, mean +/- SEM, n = 20, p less than .05). Interleukin-2 receptor expression was measured by staining with FITC-conjugated anti-CD25 antibodies and flow cytometry, following stimulation with phytohemagglutinin (PHA) or pokeweed mitogen (PWM). Perturbation of the cytoskeletal system with pentoxifylline, which has been shown to decrease F-actin content and inhibit the expression of several cell surface receptors, had similar effects on leukocytes from young and old donors.(ABSTRACT TRUNCATED AT 250 WORDS)

Duke Scholars

Published In

J Gerontol

DOI

ISSN

0022-1422

Publication Date

March 1992

Volume

47

Issue

2

Start / End Page

B37 / B44

Location

United States

Related Subject Headings

  • Receptors, Interleukin-2
  • Receptors, Immunologic
  • Receptors, Formyl Peptide
  • Peptides, Cyclic
  • Orosomucoid
  • Middle Aged
  • Macrophage-1 Antigen
  • Lymphocytes
  • Lymphocyte Activation
  • Leukocytes
 

Citation

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Rao, K. M., Currie, M. S., Padmanabhan, J., & Cohen, H. J. (1992). Age-related alterations in actin cytoskeleton and receptor expression in human leukocytes. J Gerontol, 47(2), B37–B44. https://doi.org/10.1093/geronj/47.2.b37
Rao, K. M., M. S. Currie, J. Padmanabhan, and H. J. Cohen. “Age-related alterations in actin cytoskeleton and receptor expression in human leukocytes.J Gerontol 47, no. 2 (March 1992): B37–44. https://doi.org/10.1093/geronj/47.2.b37.
Rao KM, Currie MS, Padmanabhan J, Cohen HJ. Age-related alterations in actin cytoskeleton and receptor expression in human leukocytes. J Gerontol. 1992 Mar;47(2):B37–44.
Rao, K. M., et al. “Age-related alterations in actin cytoskeleton and receptor expression in human leukocytes.J Gerontol, vol. 47, no. 2, Mar. 1992, pp. B37–44. Pubmed, doi:10.1093/geronj/47.2.b37.
Rao KM, Currie MS, Padmanabhan J, Cohen HJ. Age-related alterations in actin cytoskeleton and receptor expression in human leukocytes. J Gerontol. 1992 Mar;47(2):B37–B44.

Published In

J Gerontol

DOI

ISSN

0022-1422

Publication Date

March 1992

Volume

47

Issue

2

Start / End Page

B37 / B44

Location

United States

Related Subject Headings

  • Receptors, Interleukin-2
  • Receptors, Immunologic
  • Receptors, Formyl Peptide
  • Peptides, Cyclic
  • Orosomucoid
  • Middle Aged
  • Macrophage-1 Antigen
  • Lymphocytes
  • Lymphocyte Activation
  • Leukocytes