Preparation and characterization of unilamellar vesicles from cholate-phospholipid micelle treated with cholestyramine.

Journal Article (Journal Article)

Cholestyramine, a well-known bile-salt sequestrant, can be used effectively to remove cholate or deoxycholate from a solution of phosphatidylcholine-bile salt mixed micelle. Upon removal of the bile salt, unilamellar phospholipid vesicles form essentially instantaneously. Cholestyramine resin could be pelleted and removed from the vesicle solution after a low speed centrifugation. Based on phosphate analyses, the recovery of vesicles was approximately 60% of the starting material. The average diameter of these vesicles, as estimated by gel exclusion chromatography on sephacryl S-1000 beads and by trapped volume measurement using [3H]sucrose, ranged between 85 to 121 nm. Phosphatidylethanolamine, cholesterol, or n-alkane such as tetradecane can be incorporated into the vesicles without any selective loss; however, selective loss was experienced when negatively charged phospholipid species such as phosphatidylglycerol or phosphatidylserine was included in vesicle formation.

Full Text

Duke Authors

Cited Authors

  • Ventimiglia, JB; Levesque, MC; Chang, TY

Published Date

  • September 1986

Published In

Volume / Issue

  • 157 / 2

Start / End Page

  • 323 - 330

PubMed ID

  • 3777436

International Standard Serial Number (ISSN)

  • 0003-2697

Digital Object Identifier (DOI)

  • 10.1016/0003-2697(86)90633-0


  • eng

Conference Location

  • United States