Aerosolized manganese SOD decreases hyperoxic pulmonary injury in primates. II. Morphometric analysis.
Published
Journal Article
Hyperoxia damages lung parenchyma via increased cellular production of reactive oxygen species that exceeds antioxidant defenses. We hypothesized that aerosolized human recombinant manganese superoxide dismutase (rhMnSOD) would augment extracellular antioxidant defenses and attenuate epithelial injury in the lung during hyperoxia in primates. Twenty-four adult male baboons were anesthetized and mechanically ventilated with 100% oxygen for 96 h. The baboons were divided equally into four groups. Oxygen alone and oxygen plus rhMnSOD given at 3 mg . kg-1 . day-1 were compared to assess efficacy of the drug. Subsequently, aerosolized rhMnSOD was given at 1 or 10 mg . kg-1 . day-1 to study dose effects and toxicity. Quantitative morphometry showed protection of alveolar epithelium from hyperoxia by 3 mg . kg-1 . day-1 rhMnSOD (P < 0.05). In addition, interstitial fibroblast volumes were increased in the treatment group (P = 0.06). This effect appeared greater at the two higher doses of the rhMnSOD. The aerosolized drug was localized to the surface of airways and air spaces and macrophages by immunolabeling studies, suggesting efficacy via physicochemical properties that localize it to cell surfaces or by effects on alveolar macrophage function.
Full Text
Duke Authors
Cited Authors
- Welty-Wolf, KE; Simonson, SG; Huang, YC; Kantrow, SP; Carraway, MS; Chang, LY; Crapo, JD; Piantadosi, CA
Published Date
- August 1997
Published In
Volume / Issue
- 83 / 2
Start / End Page
- 559 - 568
PubMed ID
- 9262453
Pubmed Central ID
- 9262453
International Standard Serial Number (ISSN)
- 8750-7587
Digital Object Identifier (DOI)
- 10.1152/jappl.1997.83.2.559
Language
- eng
Conference Location
- United States