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Intrinsic activity of human immunodeficiency virus type 1 protease heterologous fusion proteins in mammalian cells.

Publication ,  Journal Article
Arrigo, SJ; Haines, JK; Huffman, KM
Published in: DNA Cell Biol
January 1995

We have generated various mammalian expression constructs that produce fusion proteins of human immunodeficiency virus type 1 (HIV-1) protease (PR) with the HIV-1 Nef protein. The expression of these proteins is inducible by the HIV-1 Tat protein. High-level expression of proteolytically active PR was produced from PR imbedded into Nef coding sequences, flanked by PR cleavage sites. The fusion protein was cleaved nearly to completion and did not exhibit the regulated processing that is seen with the virally encoded PR. No cytotoxic effect of PR expression was detected. The self-cleavage of PR could be inhibited by a specific inhibitor of HIV-1 PR (U75875). Elimination of the aminoterminal PR cleavage site did not have a measurable effect on cleavage of the precursor fusion protein. The cleaved fusion proteins appeared to be extremely unstable in the transfected cells. These findings demonstrate the intrinsic activity of HIV-1 PR in mammalian cells, in the context of a heterologous fusion protein.

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Published In

DNA Cell Biol

DOI

ISSN

1044-5498

Publication Date

January 1995

Volume

14

Issue

1

Start / End Page

15 / 23

Location

United States

Related Subject Headings

  • tat Gene Products, Human Immunodeficiency Virus
  • nef Gene Products, Human Immunodeficiency Virus
  • Sequence Deletion
  • Recombinant Fusion Proteins
  • RNA, Messenger
  • Protein Processing, Post-Translational
  • Molecular Sequence Data
  • Mammals
  • Humans
  • HIV-1
 

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Arrigo, S. J., Haines, J. K., & Huffman, K. M. (1995). Intrinsic activity of human immunodeficiency virus type 1 protease heterologous fusion proteins in mammalian cells. DNA Cell Biol, 14(1), 15–23. https://doi.org/10.1089/dna.1995.14.15
Arrigo, S. J., J. K. Haines, and K. M. Huffman. “Intrinsic activity of human immunodeficiency virus type 1 protease heterologous fusion proteins in mammalian cells.DNA Cell Biol 14, no. 1 (January 1995): 15–23. https://doi.org/10.1089/dna.1995.14.15.
Arrigo, S. J., et al. “Intrinsic activity of human immunodeficiency virus type 1 protease heterologous fusion proteins in mammalian cells.DNA Cell Biol, vol. 14, no. 1, Jan. 1995, pp. 15–23. Pubmed, doi:10.1089/dna.1995.14.15.
Journal cover image

Published In

DNA Cell Biol

DOI

ISSN

1044-5498

Publication Date

January 1995

Volume

14

Issue

1

Start / End Page

15 / 23

Location

United States

Related Subject Headings

  • tat Gene Products, Human Immunodeficiency Virus
  • nef Gene Products, Human Immunodeficiency Virus
  • Sequence Deletion
  • Recombinant Fusion Proteins
  • RNA, Messenger
  • Protein Processing, Post-Translational
  • Molecular Sequence Data
  • Mammals
  • Humans
  • HIV-1