Human mononuclear phagocyte transglutaminase activity cross-links fibrin.

Published

Journal Article

The physiologic function of the monocyte transglutaminases is not known. In this study, we detected Factor XIII A-subunit antigen and "tissue" transglutaminase antigen in human monocytes by polyacrylamide gel electrophoresis and immunoblotting techniques. Flow cytometric analysis demonstrated that 27% and 49% of the total Factor XIII antigen in monocytes and human peritoneal macrophages, respectively, are expressed on the surface of the cells. Monocytes maintained in culture for 8 days had a 4-fold increase in Factor XIIIa activity and a 3.2-fold increase in the amount of Factor XIII antigen/mg cell protein. However, there was no increase in the "tissue" transglutaminase activity or antigen levels in cultured monocytes. In addition, we identified a Factor XIII deficient individual who does not express Factor XIII activity or antigen in plasma, platelets, monocytes, lymphocytes or erythrocytes. Intact monocytes from normal donors were able to cross-link fibrin formed in the plasma from the Factor XIII deficient individual. This suggests that transglutaminase activity expressed by peripheral blood monocytes may play a physiologic role in cross-linking fibrin during blood clotting or inflammation.

Full Text

Duke Authors

Cited Authors

  • Conkling, PR; Achyuthan, KE; Greenberg, CS; Newcomb, TF; Weinberg, JB

Published Date

  • July 1, 1989

Published In

Volume / Issue

  • 55 / 1

Start / End Page

  • 57 - 68

PubMed ID

  • 2571199

Pubmed Central ID

  • 2571199

Electronic International Standard Serial Number (EISSN)

  • 1879-2472

International Standard Serial Number (ISSN)

  • 0049-3848

Digital Object Identifier (DOI)

  • 10.1016/0049-3848(89)90456-8

Language

  • eng