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Inducible nitric oxide synthase expression by peritoneal macrophages in endometriosis-associated infertility.

Publication ,  Journal Article
Osborn, BH; Haney, AF; Misukonis, MA; Weinberg, JB
Published in: Fertil Steril
January 2002

OBJECTIVE: Determine whether peritoneal macrophages from women with endometriosis-associated infertility express more inducible nitric oxide synthase (NOS2) and produce more NO than fertile controls. DESIGN: Unblinded clinical study. PATIENT(S): Nine infertile women with endometriosis and nine normal fertile women undergoing laparoscopy. INTERVENTION(S): Peritoneal fluid and macrophages were collected. Cells were also cultured with the NOS2 inducers interferon-alpha (IFN-alpha) or IFN-gamma plus lipopolysaccharide (LPS). MAIN OUTCOME MEASURE(S): Peritoneal fluid NO levels, peritoneal macrophage NOS activity, and peritoneal macrophage NOS2 protein expression. RESULT(S): NOS enzyme activity was higher in peritoneal macrophages from endometriosis patients. Immunoblots demonstrated NOS2 protein only in peritoneal macrophages from women with endometriosis. Peritoneal fluid NO concentration was similar in the two groups, but total peritoneal fluid NO content was higher in endometriosis patients. After 3 days' culture, peritoneal macrophages from women with endometriosis produced more NO in response to IFN-alpha or IFN-gamma plus LPS than controls. CONCLUSION(S): Peritoneal macrophages from women with endometriosis-associated infertility express higher levels of NOS2, have higher NOS enzyme activity, and produce more NO in response to immune stimulation in vitro. As high levels of NO adversely affect sperm, embryos, implantation, and oviductal function, reducing peritoneal fluid NO production or blocking NO effects may improve fertility in women with endometriosis.

Duke Scholars

Published In

Fertil Steril

DOI

ISSN

0015-0282

Publication Date

January 2002

Volume

77

Issue

1

Start / End Page

46 / 51

Location

United States

Related Subject Headings

  • Reference Values
  • Obstetrics & Reproductive Medicine
  • Nitrites
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Synthase
  • Nitrates
  • Mice
  • Macrophages, Peritoneal
  • Macrophage Activation
  • Lipopolysaccharides
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Osborn, B. H., Haney, A. F., Misukonis, M. A., & Weinberg, J. B. (2002). Inducible nitric oxide synthase expression by peritoneal macrophages in endometriosis-associated infertility. Fertil Steril, 77(1), 46–51. https://doi.org/10.1016/s0015-0282(01)02940-5
Osborn, Barbara H., A. F. Haney, Mary A. Misukonis, and J Brice Weinberg. “Inducible nitric oxide synthase expression by peritoneal macrophages in endometriosis-associated infertility.Fertil Steril 77, no. 1 (January 2002): 46–51. https://doi.org/10.1016/s0015-0282(01)02940-5.
Osborn BH, Haney AF, Misukonis MA, Weinberg JB. Inducible nitric oxide synthase expression by peritoneal macrophages in endometriosis-associated infertility. Fertil Steril. 2002 Jan;77(1):46–51.
Osborn, Barbara H., et al. “Inducible nitric oxide synthase expression by peritoneal macrophages in endometriosis-associated infertility.Fertil Steril, vol. 77, no. 1, Jan. 2002, pp. 46–51. Pubmed, doi:10.1016/s0015-0282(01)02940-5.
Osborn BH, Haney AF, Misukonis MA, Weinberg JB. Inducible nitric oxide synthase expression by peritoneal macrophages in endometriosis-associated infertility. Fertil Steril. 2002 Jan;77(1):46–51.
Journal cover image

Published In

Fertil Steril

DOI

ISSN

0015-0282

Publication Date

January 2002

Volume

77

Issue

1

Start / End Page

46 / 51

Location

United States

Related Subject Headings

  • Reference Values
  • Obstetrics & Reproductive Medicine
  • Nitrites
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Synthase
  • Nitrates
  • Mice
  • Macrophages, Peritoneal
  • Macrophage Activation
  • Lipopolysaccharides