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IL-4 and interferon gamma regulate expression of inducible nitric oxide synthase in chronic lymphocytic leukemia cells.

Publication ,  Journal Article
Levesque, MC; Misukonis, MA; O'Loughlin, CW; Chen, Y; Beasley, BE; Wilson, DL; Adams, DJ; Silber, R; Weinberg, JB
Published in: Leukemia
February 2003

Chronic lymphocytic leukemia (B-CLL) is characterized by the accumulation of long-lived non-dividing CD5(+) B cells. Nitric oxide (NO) is an important regulator of apoptosis, and the viability of cultured B-CLL cells may be dependent on the autocrine production of nitric oxide by inducible nitric oxide synthase (NOS2). We performed this study to determine whether cytokine factors that prevent spontaneous in vitroapoptosis of B-CLL cells induce B-CLL cell NOS2 enzyme activity. B-CLL cells expressed NOS enzyme activity and NOS2 protein and mRNA. IL-4 and IFN-gamma increased B-CLL cell NOS2 enzyme activity and protein expression during in vitro culture. IFN-gamma, but not IL-4, increased NOS2 mRNA expression in cultured B-CLL cells suggesting that IL-4-mediated changes of NOS2 protein expression occurred at the post-transcriptional level. We were unable to detect increased concentrations of nitrite or nitrate (NO(x)) as surrogate markers of NO production in B-CLL cell cultures treated with IL-4 or IFN-gamma. IL-4 and IFN-gamma diminished NOS inhibitor-induced B-CLL cell death. In summary, we found that B-CLL cells expressed NOS2 and that IL-4 and IFN-gamma increased B-CLL NOS2 expression. Cytokine-mediated expression of NOS2 by B-CLL cells may promote their survival, and therapeutic strategies that target NOS2 or quench NO may be beneficial in patients with B-CLL.

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Published In

Leukemia

DOI

ISSN

0887-6924

Publication Date

February 2003

Volume

17

Issue

2

Start / End Page

442 / 450

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Nitric Oxide Synthase Type III
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Synthase
  • Leukemia, Myeloid
  • Leukemia, Lymphocytic, Chronic, B-Cell
  • Interleukin-4
  • Immunology
 

Citation

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Levesque, M. C., Misukonis, M. A., O’Loughlin, C. W., Chen, Y., Beasley, B. E., Wilson, D. L., … Weinberg, J. B. (2003). IL-4 and interferon gamma regulate expression of inducible nitric oxide synthase in chronic lymphocytic leukemia cells. Leukemia, 17(2), 442–450. https://doi.org/10.1038/sj.leu.2402783
Levesque, M. C., M. A. Misukonis, C. W. O’Loughlin, Y. Chen, B. E. Beasley, D. L. Wilson, D. J. Adams, R. Silber, and J. B. Weinberg. “IL-4 and interferon gamma regulate expression of inducible nitric oxide synthase in chronic lymphocytic leukemia cells.Leukemia 17, no. 2 (February 2003): 442–50. https://doi.org/10.1038/sj.leu.2402783.
Levesque MC, Misukonis MA, O’Loughlin CW, Chen Y, Beasley BE, Wilson DL, et al. IL-4 and interferon gamma regulate expression of inducible nitric oxide synthase in chronic lymphocytic leukemia cells. Leukemia. 2003 Feb;17(2):442–50.
Levesque, M. C., et al. “IL-4 and interferon gamma regulate expression of inducible nitric oxide synthase in chronic lymphocytic leukemia cells.Leukemia, vol. 17, no. 2, Feb. 2003, pp. 442–50. Pubmed, doi:10.1038/sj.leu.2402783.
Levesque MC, Misukonis MA, O’Loughlin CW, Chen Y, Beasley BE, Wilson DL, Adams DJ, Silber R, Weinberg JB. IL-4 and interferon gamma regulate expression of inducible nitric oxide synthase in chronic lymphocytic leukemia cells. Leukemia. 2003 Feb;17(2):442–450.

Published In

Leukemia

DOI

ISSN

0887-6924

Publication Date

February 2003

Volume

17

Issue

2

Start / End Page

442 / 450

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Nitric Oxide Synthase Type III
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Synthase
  • Leukemia, Myeloid
  • Leukemia, Lymphocytic, Chronic, B-Cell
  • Interleukin-4
  • Immunology