Human immunodeficiency virus type 1 infection of human monocytes and macrophages does not alter their ability to generate an oxidative burst.
Journal Article (Journal Article)
Human immunodeficiency virus type 1 (HIV-1) infects mononuclear phagocytes, cells that may serve as a reservoir for viral persistence. Infection with HIV-1 leads to progressive compromise of the immune system, resulting in infections with opportunistic pathogens and eventual death. Experiments were designed to determine if in vitro HIV-1 infection of mononuclear phagocytes would diminish their oxidative capabilities, thus decreasing their antimicrobial effectiveness. Blood monocytes and peritoneal macrophages were obtained from uninfected donors and inoculated with a monocytotropic strain of HIV-1. Hydrogen peroxide production and reduction of nitroblue tetrazolium were measured after acute stimulation of cells with PMA or a phagocytic stimulus. Despite vigorous virus production, no difference was seen in oxidative burst between uninfected cells and infected cells or between monocyte-derived and peritoneal macrophages. In conclusion, reduced antimicrobial activity of HIV-infected mononuclear phagocytes is probably not secondary to decreased ability to generate reactive oxygen species.
Full Text
Duke Authors
Cited Authors
- Dukes, CS; Matthews, TJ; Weinberg, JB
Published Date
- August 1993
Published In
Volume / Issue
- 168 / 2
Start / End Page
- 459 - 462
PubMed ID
- 8335985
Pubmed Central ID
- 8335985
International Standard Serial Number (ISSN)
- 0022-1899
Digital Object Identifier (DOI)
- 10.1093/infdis/168.2.459
Language
- eng
Conference Location
- United States