Nitric oxide modulation of human leukemia cell differentiation and gene expression.

Published

Journal Article

Nitric oxide (NO) functions as an intercellular messenger molecule in such varied contexts as neurotransmission, immune regulation, and the control of vascular tone. We report that NO, delivered as purified gas or released from the pharmacologic NO donors sodium nitroprusside or 6-morpholino-sydnonimine, caused monocytic differentiation of cells of the human myeloid leukemia cell line HL-60 and altered gene expression. The treated cells stopped proliferating, became spread and vacuolated, had increased expression of nonspecific esterase and the monocyte marker CD14, and displayed increased capacity to produce hydrogen peroxide. Furthermore, these treated cells had increased steady-state expression of messenger RNA (mRNA) for tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta), but decreased expression of mRNA for the proto-oncogenes c-myc and c-myb. The increase in TNF-alpha and IL-1 beta mRNA levels was due (at least in part) to a new transcription of these specific mRNAs. NO elaborated in the bone marrow microenvironment may have a role in normal and malignant hematopoietic cell growth and differentiation.

Full Text

Duke Authors

Cited Authors

  • Magrinat, G; Mason, SN; Shami, PJ; Weinberg, JB

Published Date

  • October 15, 1992

Published In

Volume / Issue

  • 80 / 8

Start / End Page

  • 1880 - 1884

PubMed ID

  • 1382708

Pubmed Central ID

  • 1382708

International Standard Serial Number (ISSN)

  • 0006-4971

Language

  • eng

Conference Location

  • United States