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Inducible nitric oxide synthase (NOS2) promoter CCTTT repeat polymorphism: relationship to in vivo nitric oxide production/NOS activity in an asymptomatic malaria-endemic population.

Publication ,  Journal Article
Boutlis, CS; Hobbs, MR; Marsh, RL; Misukonis, MA; Tkachuk, AN; Lagog, M; Booth, J; Granger, DL; Bockarie, MJ; Mgone, CS; Levesque, MC ...
Published in: Am J Trop Med Hyg
December 2003

Polymorphisms in the inducible nitric oxide synthase gene (NOS2) promoter have been associated with clinical outcome from malaria. These include a CCTTT repeat (CCTTTn) 2.5 kilobases upstream from the NOS2 transcription start site, and two single nucleotide substitutions: G-->C at position -954 (G-954C), and C-->T at position -1173 (C-1173T). Although hypothesized to influence NO production in vivo, the functional relevance of (CCTTT)n and G-954C is uncertain because disease association studies have yielded inconsistent results. This study found no association between CCTTT repeat number and levels of plasma NO metabolites or peripheral blood mononuclear cell NOS activity in a cohort of asymptomatic malaria-exposed coastal Papua New Guineans 1-60 years old. This suggests that (CCTTT)n does not independently influence NOS2 transcription in vivo. Neither the G-954C nor the C-1173T polymorphisms were identified in this population, indicating the variability and complexity of selection for NOS2 promoter polymorphisms in different malaria-endemic populations.

Duke Scholars

Published In

Am J Trop Med Hyg

ISSN

0002-9637

Publication Date

December 2003

Volume

69

Issue

6

Start / End Page

569 / 573

Location

United States

Related Subject Headings

  • Tropical Medicine
  • Repetitive Sequences, Nucleic Acid
  • Promoter Regions, Genetic
  • Polymorphism, Genetic
  • Papua New Guinea
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Synthase
  • Nitric Oxide
  • Native Hawaiian or Other Pacific Islander
  • Male
 

Citation

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MLA
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Boutlis, C. S., Hobbs, M. R., Marsh, R. L., Misukonis, M. A., Tkachuk, A. N., Lagog, M., … Anstey, N. M. (2003). Inducible nitric oxide synthase (NOS2) promoter CCTTT repeat polymorphism: relationship to in vivo nitric oxide production/NOS activity in an asymptomatic malaria-endemic population. Am J Trop Med Hyg, 69(6), 569–573.
Boutlis, Craig S., Maurine R. Hobbs, Robyn L. Marsh, Mary A. Misukonis, Ariana N. Tkachuk, Moses Lagog, Jennifer Booth, et al. “Inducible nitric oxide synthase (NOS2) promoter CCTTT repeat polymorphism: relationship to in vivo nitric oxide production/NOS activity in an asymptomatic malaria-endemic population.Am J Trop Med Hyg 69, no. 6 (December 2003): 569–73.
Boutlis CS, Hobbs MR, Marsh RL, Misukonis MA, Tkachuk AN, Lagog M, Booth J, Granger DL, Bockarie MJ, Mgone CS, Levesque MC, Weinberg JB, Anstey NM. Inducible nitric oxide synthase (NOS2) promoter CCTTT repeat polymorphism: relationship to in vivo nitric oxide production/NOS activity in an asymptomatic malaria-endemic population. Am J Trop Med Hyg. 2003 Dec;69(6):569–573.

Published In

Am J Trop Med Hyg

ISSN

0002-9637

Publication Date

December 2003

Volume

69

Issue

6

Start / End Page

569 / 573

Location

United States

Related Subject Headings

  • Tropical Medicine
  • Repetitive Sequences, Nucleic Acid
  • Promoter Regions, Genetic
  • Polymorphism, Genetic
  • Papua New Guinea
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Synthase
  • Nitric Oxide
  • Native Hawaiian or Other Pacific Islander
  • Male