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Stimulation of ovarian tumor cell proliferation with monocyte products including interleukin-1, interleukin-6, and tumor necrosis factor-alpha.

Publication ,  Journal Article
Wu, S; Rodabaugh, K; Martinez-Maza, O; Watson, JM; Silberstein, DS; Boyer, CM; Peters, WP; Weinberg, JB; Berek, JS; Bast, RC
Published in: Am J Obstet Gynecol
March 1992

OBJECTIVE: We investigated whether monocyte-derived factors could stimulate the growth of ovarian cancer cells. STUDY DESIGN: Human peripheral blood monocytes or human monocyte-like cell lines THP-1 and U-937 were cultured with or without macrophage colony-stimulating factor, lipopolysaccharide, or phorbol myristate acetate. Culture supernatants or recombinant cytokines were assayed for growth stimulation of ovarian cancer cell lines by tritium-thymidine incorporation and direct cell counts followed by statistical analysis with Student t test. RESULTS: Conditioned medium from peripheral blood monocytes or from THP-1 or U-937 cells stimulated ovarian cancer cell growth. Interleukin-1 alpha, tumor necrosis factor-alpha, and interleukin-6 also stimulated ovarian cancer cell growth, whereas macrophage, granulocyte, and granulocyte-macrophage colony-stimulating factor did not. Concentrations of tumor necrosis factor, interleukin-1, and interleukin-6 in conditioned medium could not account for all the growth stimulation, and activity remained after neutralization of tumor necrosis factor, interleukin-1, and interleukin-6 with antibodies. CONCLUSIONS: Interleukin-1, interleukin-6, tumor necrosis factor, and additional monocyte factor(s) could provide paracrine growth stimulation when monocytes are attracted to ovarian cancers that produce macrophage colony-stimulating factor.

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Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

March 1992

Volume

166

Issue

3

Start / End Page

997 / 1007

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Tetradecanoylphorbol Acetate
  • Ovarian Neoplasms
  • Obstetrics & Reproductive Medicine
  • Monocytes
  • Lipopolysaccharides
  • Interleukin-6
  • Interleukin-1
  • Humans
 

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Wu, S., Rodabaugh, K., Martinez-Maza, O., Watson, J. M., Silberstein, D. S., Boyer, C. M., … Bast, R. C. (1992). Stimulation of ovarian tumor cell proliferation with monocyte products including interleukin-1, interleukin-6, and tumor necrosis factor-alpha. Am J Obstet Gynecol, 166(3), 997–1007. https://doi.org/10.1016/0002-9378(92)91379-o
Wu, S., K. Rodabaugh, O. Martinez-Maza, J. M. Watson, D. S. Silberstein, C. M. Boyer, W. P. Peters, J. B. Weinberg, J. S. Berek, and R. C. Bast. “Stimulation of ovarian tumor cell proliferation with monocyte products including interleukin-1, interleukin-6, and tumor necrosis factor-alpha.Am J Obstet Gynecol 166, no. 3 (March 1992): 997–1007. https://doi.org/10.1016/0002-9378(92)91379-o.
Wu S, Rodabaugh K, Martinez-Maza O, Watson JM, Silberstein DS, Boyer CM, et al. Stimulation of ovarian tumor cell proliferation with monocyte products including interleukin-1, interleukin-6, and tumor necrosis factor-alpha. Am J Obstet Gynecol. 1992 Mar;166(3):997–1007.
Wu, S., et al. “Stimulation of ovarian tumor cell proliferation with monocyte products including interleukin-1, interleukin-6, and tumor necrosis factor-alpha.Am J Obstet Gynecol, vol. 166, no. 3, Mar. 1992, pp. 997–1007. Pubmed, doi:10.1016/0002-9378(92)91379-o.
Wu S, Rodabaugh K, Martinez-Maza O, Watson JM, Silberstein DS, Boyer CM, Peters WP, Weinberg JB, Berek JS, Bast RC. Stimulation of ovarian tumor cell proliferation with monocyte products including interleukin-1, interleukin-6, and tumor necrosis factor-alpha. Am J Obstet Gynecol. 1992 Mar;166(3):997–1007.
Journal cover image

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

March 1992

Volume

166

Issue

3

Start / End Page

997 / 1007

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Tetradecanoylphorbol Acetate
  • Ovarian Neoplasms
  • Obstetrics & Reproductive Medicine
  • Monocytes
  • Lipopolysaccharides
  • Interleukin-6
  • Interleukin-1
  • Humans