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Vacuolar and plasma membrane stripping and autophagic elimination of Toxoplasma gondii in primed effector macrophages.

Publication ,  Journal Article
Ling, YM; Shaw, MH; Ayala, C; Coppens, I; Taylor, GA; Ferguson, DJP; Yap, GS
Published in: J Exp Med
September 4, 2006

Apicomplexan protozoan pathogens avoid destruction and establish a replicative niche within host cells by forming a nonfusogenic parasitophorous vacuole (PV). Here we present evidence for lysosome-mediated degradation of Toxoplasma gondii after invasion of macrophages activated in vivo. Pathogen elimination was dependent on the interferon gamma inducible-p47 GTPase, IGTP, required PI3K activity, and was preceded by PV membrane indentation, vesiculation, disruption, and, surprisingly, stripping of the parasite plasma membrane. Denuded parasites were enveloped in autophagosome-like vacuoles, which ultimately fused with lysosomes. These observations outline a series of mechanisms used by effector cells to redirect the fate of a classically nonfusogenic intracellular pathogen toward a path of immune elimination.

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Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

September 4, 2006

Volume

203

Issue

9

Start / End Page

2063 / 2071

Location

United States

Related Subject Headings

  • Vacuoles
  • Toxoplasma
  • Phosphoinositide-3 Kinase Inhibitors
  • Phosphatidylinositol 3-Kinases
  • Mice, Knockout
  • Mice, Inbred Strains
  • Mice
  • Membrane Fusion
  • Macrophages
  • Lysosomes
 

Citation

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Ling, Y. M., Shaw, M. H., Ayala, C., Coppens, I., Taylor, G. A., Ferguson, D. J. P., & Yap, G. S. (2006). Vacuolar and plasma membrane stripping and autophagic elimination of Toxoplasma gondii in primed effector macrophages. J Exp Med, 203(9), 2063–2071. https://doi.org/10.1084/jem.20061318
Ling, Yun M., Michael H. Shaw, Carol Ayala, Isabelle Coppens, Gregory A. Taylor, David J. P. Ferguson, and George S. Yap. “Vacuolar and plasma membrane stripping and autophagic elimination of Toxoplasma gondii in primed effector macrophages.J Exp Med 203, no. 9 (September 4, 2006): 2063–71. https://doi.org/10.1084/jem.20061318.
Ling YM, Shaw MH, Ayala C, Coppens I, Taylor GA, Ferguson DJP, et al. Vacuolar and plasma membrane stripping and autophagic elimination of Toxoplasma gondii in primed effector macrophages. J Exp Med. 2006 Sep 4;203(9):2063–71.
Ling, Yun M., et al. “Vacuolar and plasma membrane stripping and autophagic elimination of Toxoplasma gondii in primed effector macrophages.J Exp Med, vol. 203, no. 9, Sept. 2006, pp. 2063–71. Pubmed, doi:10.1084/jem.20061318.
Ling YM, Shaw MH, Ayala C, Coppens I, Taylor GA, Ferguson DJP, Yap GS. Vacuolar and plasma membrane stripping and autophagic elimination of Toxoplasma gondii in primed effector macrophages. J Exp Med. 2006 Sep 4;203(9):2063–2071.

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

September 4, 2006

Volume

203

Issue

9

Start / End Page

2063 / 2071

Location

United States

Related Subject Headings

  • Vacuoles
  • Toxoplasma
  • Phosphoinositide-3 Kinase Inhibitors
  • Phosphatidylinositol 3-Kinases
  • Mice, Knockout
  • Mice, Inbred Strains
  • Mice
  • Membrane Fusion
  • Macrophages
  • Lysosomes