Skip to main content

Gamma interferon-induced inhibition of Toxoplasma gondii in astrocytes is mediated by IGTP.

Publication ,  Journal Article
Halonen, SK; Taylor, GA; Weiss, LM
Published in: Infect Immun
September 2001

Toxoplasma gondii is an important pathogen in the central nervous system, causing a severe and often fatal encephalitis in patients with AIDS. Gamma interferon (IFN-gamma) is the main cytokine preventing reactivation of Toxoplasma encephalitis in the brain. Microglia are important IFN-gamma-activated effector cells controlling the growth of T. gondii in the brain via a nitric oxide (NO)-mediated mechanism. IFN-gamma can also activate astrocytes to inhibit the growth of T. gondii. Previous studies found that the mechanism in murine astrocytes is independent of NO and all other known anti-Toxoplasma mechanisms. In this study we investigated the role of IGTP, a recently identified IFN-gamma-regulated gene, in IFN-gamma inhibition of T. gondii in murine astrocytes. Primary astrocytes were cultivated from IGTP-deficient mice, treated with IFN-gamma, and then tested for anti-Toxoplasma activity. In wild-type astrocytes T. gondii growth was significantly inhibited by IFN-gamma, whereas in astrocytes from IGTP-deficient mice IFN-gamma did not cause a significant inhibition of growth. Immunoblot analysis confirmed that IFN-gamma induced significant levels of IGTP in wild-type murine astrocytes within 24 h. These results indicate that IGTP plays a central role in the IFN-gamma-induced inhibition of T. gondii in murine astrocytes.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Infect Immun

DOI

ISSN

0019-9567

Publication Date

September 2001

Volume

69

Issue

9

Start / End Page

5573 / 5576

Location

United States

Related Subject Headings

  • Toxoplasmosis, Cerebral
  • Toxoplasma
  • Microbiology
  • Mice, Inbred C57BL
  • Mice
  • Interferon-gamma
  • GTP Phosphohydrolases
  • Cells, Cultured
  • Astrocytes
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Halonen, S. K., Taylor, G. A., & Weiss, L. M. (2001). Gamma interferon-induced inhibition of Toxoplasma gondii in astrocytes is mediated by IGTP. Infect Immun, 69(9), 5573–5576. https://doi.org/10.1128/IAI.69.9.5573-5576.2001
Halonen, S. K., G. A. Taylor, and L. M. Weiss. “Gamma interferon-induced inhibition of Toxoplasma gondii in astrocytes is mediated by IGTP.Infect Immun 69, no. 9 (September 2001): 5573–76. https://doi.org/10.1128/IAI.69.9.5573-5576.2001.
Halonen SK, Taylor GA, Weiss LM. Gamma interferon-induced inhibition of Toxoplasma gondii in astrocytes is mediated by IGTP. Infect Immun. 2001 Sep;69(9):5573–6.
Halonen, S. K., et al. “Gamma interferon-induced inhibition of Toxoplasma gondii in astrocytes is mediated by IGTP.Infect Immun, vol. 69, no. 9, Sept. 2001, pp. 5573–76. Pubmed, doi:10.1128/IAI.69.9.5573-5576.2001.
Halonen SK, Taylor GA, Weiss LM. Gamma interferon-induced inhibition of Toxoplasma gondii in astrocytes is mediated by IGTP. Infect Immun. 2001 Sep;69(9):5573–5576.

Published In

Infect Immun

DOI

ISSN

0019-9567

Publication Date

September 2001

Volume

69

Issue

9

Start / End Page

5573 / 5576

Location

United States

Related Subject Headings

  • Toxoplasmosis, Cerebral
  • Toxoplasma
  • Microbiology
  • Mice, Inbred C57BL
  • Mice
  • Interferon-gamma
  • GTP Phosphohydrolases
  • Cells, Cultured
  • Astrocytes
  • Animals