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Regulation of P311 expression by Met-hepatocyte growth factor/scatter factor and the ubiquitin/proteasome system.

Publication ,  Journal Article
Taylor, GA; Hudson, E; Resau, JH; Vande Woude, GF
Published in: J Biol Chem
February 11, 2000

P311 is a mouse cDNA originally identified for its high expression in late-stage embryonic brain and adult cerebellum, hippocampus, and olfactory bulb. The protein product of P311, however, had not been identified previously, and its function remains unknown. We report here that P311 expression is regulated at multiple levels by pathways that control cellular transformation. P311 mRNA expression was decreased sharply in both neural and smooth muscle cells when the cells were transformed by coexpression of the oncogenic tyrosine kinase receptor Met and its ligand hepatocyte growth factor/scatter factor. The P311 mRNA was found to encode an 8-kDa polypeptide that was subject to rapid degradation by the lactacystin-sensitive ubiquitin/proteasome system and an unidentified metalloprotease, resulting in a protein half-life of about 5 min. These data suggest that P311 expression is dramatically decreased by several pathways that regulate cellular growth.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

February 11, 2000

Volume

275

Issue

6

Start / End Page

4215 / 4219

Location

United States

Related Subject Headings

  • Ubiquitins
  • Transformation, Genetic
  • Receptor Protein-Tyrosine Kinases
  • RNA, Messenger
  • Proteasome Endopeptidase Complex
  • Phenanthrolines
  • Nerve Tissue Proteins
  • Muscle, Smooth
  • Multienzyme Complexes
  • Mice, Nude
 

Citation

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Taylor, G. A., Hudson, E., Resau, J. H., & Vande Woude, G. F. (2000). Regulation of P311 expression by Met-hepatocyte growth factor/scatter factor and the ubiquitin/proteasome system. J Biol Chem, 275(6), 4215–4219. https://doi.org/10.1074/jbc.275.6.4215
Taylor, G. A., E. Hudson, J. H. Resau, and G. F. Vande Woude. “Regulation of P311 expression by Met-hepatocyte growth factor/scatter factor and the ubiquitin/proteasome system.J Biol Chem 275, no. 6 (February 11, 2000): 4215–19. https://doi.org/10.1074/jbc.275.6.4215.
Taylor GA, Hudson E, Resau JH, Vande Woude GF. Regulation of P311 expression by Met-hepatocyte growth factor/scatter factor and the ubiquitin/proteasome system. J Biol Chem. 2000 Feb 11;275(6):4215–9.
Taylor, G. A., et al. “Regulation of P311 expression by Met-hepatocyte growth factor/scatter factor and the ubiquitin/proteasome system.J Biol Chem, vol. 275, no. 6, Feb. 2000, pp. 4215–19. Pubmed, doi:10.1074/jbc.275.6.4215.
Taylor GA, Hudson E, Resau JH, Vande Woude GF. Regulation of P311 expression by Met-hepatocyte growth factor/scatter factor and the ubiquitin/proteasome system. J Biol Chem. 2000 Feb 11;275(6):4215–4219.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

February 11, 2000

Volume

275

Issue

6

Start / End Page

4215 / 4219

Location

United States

Related Subject Headings

  • Ubiquitins
  • Transformation, Genetic
  • Receptor Protein-Tyrosine Kinases
  • RNA, Messenger
  • Proteasome Endopeptidase Complex
  • Phenanthrolines
  • Nerve Tissue Proteins
  • Muscle, Smooth
  • Multienzyme Complexes
  • Mice, Nude